COMPARISON OF IGE-REACTIVITY OF ASPERGILLUS FUMIGATUS, ASPERGILLUS FLAVOUS AND ASPERGILLUS NIGER WITH TYPE 1 HYPERSENSITIVE PATIENTS’ SERA

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FALAHATI MEHRABAN; EBRAHIMI MOJHGAN; SALEK MOGHADAM ALI REZA; ROUDBARY MARYAM; GHANBARI SOMAYEH; Mokhtarian Kobra; KHOSHMIRSAFA MAJID; FALAK REZA

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Journal: Razi Journal of Medical Sciences Year:2017 | Volume:23 | Issue:151 Page(s): 10-20

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Title

COMPARISON OF IGE-REACTIVITY OF ASPERGILLUS FUMIGATUS, ASPERGILLUS FLAVOUS AND ASPERGILLUS NIGER WITH TYPE 1 HYPERSENSITIVE PATIENTS’ SERA

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Keywords

TYPE 1 HYPER-SENSITIVITYQ1

ASPERGILLUS SPECIESQ1

WESTERN BLOTTINGQ2

ELISAQ3

Abstract

Background: ASPERGILLUS SPECIES have crucial role in respiratory allergy. This study was aimed to evaluate the pattern of IgE-mediated immune response to common ASPERGILLUS SPECIES including Aspergillus fumigatus, Aspergillus flavous, Aspergillus niger in type I aspergillus-hypersensitive patients’ sera.Methods: In this experimental study, the prepared protein extract of ASPERGILLUS SPECIES were dialyzed and the protein concentrations were measured by Bradford method. The protein patterns were checked by SDS-PAGE. The allergic patients and controls were selected based on their clinical history and skin prick test results. The cross-reactivity of allergens were determined by ELISA and Western-blotting using allergic patients’ and controls’ sera.Results: SDS-PAGE showed that the molecular weight of most of aspergillus proteins are between 11-100 kDa with typical bands between 46-100 kDa. ELISA showed that there is a significant difference between the serum-immunoreactivity of patients and negative controls for each of studied species. However, we did not find significant difference between the immunoreactivity of the patients with the studied fungal species. Western-blotting also detected various typical IgE-reactive proteins for each of ASPERGILLUS SPECIES.Conclusion: The IgE-reactive pattern of ASPERGILLUS SPECIES showed a slight difference. The variation of the immunoreactivity of the ASPERGILLUS SPECIES could be due to the genetic variations, difference in the environmental predisposition with saprophyte molds or non-equal allergenic potency of the extracts.

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APA: Copy

FALAHATI, MEHRABAN, EBRAHIMI, MOJHGAN, SALEK MOGHADAM, ALI REZA, ROUDBARY, MARYAM, GHANBARI, SOMAYEH, Mokhtarian, Kobra, KHOSHMIRSAFA, MAJID, & FALAK, REZA. (2017). COMPARISON OF IGE-REACTIVITY OF ASPERGILLUS FUMIGATUS, ASPERGILLUS FLAVOUS AND ASPERGILLUS NIGER WITH TYPE 1 HYPERSENSITIVE PATIENTS’ SERA. RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES), 23(151), 10-20. SID. https://sid.ir/paper/10558/en

Vancouver: Copy

FALAHATI MEHRABAN, EBRAHIMI MOJHGAN, SALEK MOGHADAM ALI REZA, ROUDBARY MARYAM, GHANBARI SOMAYEH, Mokhtarian Kobra, KHOSHMIRSAFA MAJID, FALAK REZA. COMPARISON OF IGE-REACTIVITY OF ASPERGILLUS FUMIGATUS, ASPERGILLUS FLAVOUS AND ASPERGILLUS NIGER WITH TYPE 1 HYPERSENSITIVE PATIENTS’ SERA. RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES)[Internet]. 2017;23(151):10-20. Available from: https://sid.ir/paper/10558/en

IEEE: Copy

MEHRABAN FALAHATI, MOJHGAN EBRAHIMI, ALI REZA SALEK MOGHADAM, MARYAM ROUDBARY, SOMAYEH GHANBARI, Kobra Mokhtarian, MAJID KHOSHMIRSAFA, and REZA FALAK, “COMPARISON OF IGE-REACTIVITY OF ASPERGILLUS FUMIGATUS, ASPERGILLUS FLAVOUS AND ASPERGILLUS NIGER WITH TYPE 1 HYPERSENSITIVE PATIENTS’ SERA,” RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES), vol. 23, no. 151, pp. 10–20, 2017, [Online]. Available: https://sid.ir/paper/10558/en

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