CRYOPRESERVATION METHODS FOR DIFFERENT SPECIES OF LEISHMANIA

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KOUSHA S.; MOHEBALI M.

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Journal: Researcher Bulletin of Medical Sciences Year:2000 | Volume:5 | Issue:2 (18) Page(s): 157-164

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Title

CRYOPRESERVATION METHODS FOR DIFFERENT SPECIES OF LEISHMANIA

Author(s)

KOUSHA S. | MOHEBALI M. | Issue Writer Certificate

Keywords

LEISHMANIAQ3

CRYOPRESERVATIONQ3

Abstract

LEISHMANIA is a common parasitic protozoan in Iran. This genus includes more than 20 species, each of them having several strains. Cutaneous and visceral LEISHMANIAsis are caused by the microorganisms of this group and are spread in an endemic and sporadic form. Considering the severe and threatening complications of some kinds of LEISHMANIAsis in human population, it is very important to preserve safely these protozoa in labs for a long time for preparation of vaccines, and in addition to study its species and strains. Conventional methods for preservation and maintenance of this protozoan are in-vitro culture or performing passage in sensitive animals. These procedures are rather expensive, time-consuming, and with a high risk of human contamination through culture media. In addition, it is possible that antigenic, biologic, and chemical properties of microorganisms alter in culture media. Therefore, it is preferably recommended to use CRYOPRESERVATION method. The explorative and descriptive strategy of this study was carried out in parasitologic lab of Health School and Health Research Institute of Tehran Uni. of Med. Sci. In this respect, 4 species and strains of LEISHMANIA (responsible for Kalaazar and cutaneous LEISHMANIAsis) were studied and cryopreserved for one year using DMSO (Dimethyl sulfuxide). It is concluded that CRYOPRESERVATION is more effective than the other available method and it is appropriate to be used for LEISHMANIA.

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APA: Copy

KOUSHA, S., & MOHEBALI, M.. (2000). CRYOPRESERVATION METHODS FOR DIFFERENT SPECIES OF LEISHMANIA. RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH), 5(2 (18)), 157-164. SID. https://sid.ir/paper/18230/en

Vancouver: Copy

KOUSHA S., MOHEBALI M.. CRYOPRESERVATION METHODS FOR DIFFERENT SPECIES OF LEISHMANIA. RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH)[Internet]. 2000;5(2 (18)):157-164. Available from: https://sid.ir/paper/18230/en

IEEE: Copy

S. KOUSHA, and M. MOHEBALI, “CRYOPRESERVATION METHODS FOR DIFFERENT SPECIES OF LEISHMANIA,” RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH), vol. 5, no. 2 (18), pp. 157–164, 2000, [Online]. Available: https://sid.ir/paper/18230/en

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