COMPARSION BETWEEN CATION-EXCHANGE CHROMATOGRAPHY USING HBGOLD IVD AND REFERENCE METHOD OF MICRO-CHROMATOGRAPHY IN QUALIFICATIVE MEASUREMENT OF HEMOGLOBIN A2

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MOHAMMADI TORBATI P.; RASTEGAR RAD N.

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Journal: Researcher Bulletin of Medical Sciences Year:2007 | Volume:12 | Issue:4 (58) Page(s): 329-338

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Title

COMPARSION BETWEEN CATION-EXCHANGE CHROMATOGRAPHY USING HBGOLD IVD AND REFERENCE METHOD OF MICRO-CHROMATOGRAPHY IN QUALIFICATIVE MEASUREMENT OF HEMOGLOBIN A2

Author(s)

MOHAMMADI TORBATI P. | RASTEGAR RAD N. | Issue Writer Certificate

Keywords

HBA2Q3

CATION EXCHANGE CHROMATOGRAPHYQ3

MICRO CHROMATOGRAPHYQ3

HBGOLDQ3

Abstract

Background: Accurate and precise hemoglobin separation and quantitation of HbA2 and HbF are essential for diagnosis of thalassemia. To evaluate the potential application of cation-exchange chromatography by HBGOLD IVD in performing hemoglobin A2 quantificative assay, comparison between this technique and reference method of micro-chromatography was carried out. Materials and Methods: The blood samples were obtained from individuals who had either thalassemia trait or healthy status (n=321).Results: Variability of HbA2 quantitation was quite low; the CVs of within-run between run & interlaboratory studies were: 1.8-3.1% , 3.4-6% and 6.6-8.8%, respectively. The results of bA2% quantitated by HBGOLD analyzer correlated with those given by Helena microchromatogrophy system (r=0.974.p=0.001).Conclusion: The HBGOLD was simple, rapid and reproducible. The application of HBGOLD analyzer for disgnosis of various phenotypes of thalassemia (the ailment which is frequently observed in Iran) is considered. In conclusion the HbA2 assay of HBGOLD analyzer could be used for both the quantition of HbA2 and the presumptive identification of thalassemia.

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APA: Copy

MOHAMMADI TORBATI, P., & RASTEGAR RAD, N.. (2007). COMPARSION BETWEEN CATION-EXCHANGE CHROMATOGRAPHY USING HBGOLD IVD AND REFERENCE METHOD OF MICRO-CHROMATOGRAPHY IN QUALIFICATIVE MEASUREMENT OF HEMOGLOBIN A_{2. RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH), 12(4 (58)), 329-338. SID. https://sid.ir/paper/18544/en}

Vancouver: Copy

MOHAMMADI TORBATI P., RASTEGAR RAD N.. COMPARSION BETWEEN CATION-EXCHANGE CHROMATOGRAPHY USING HBGOLD IVD AND REFERENCE METHOD OF MICRO-CHROMATOGRAPHY IN QUALIFICATIVE MEASUREMENT OF HEMOGLOBIN A_{2. RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH)[Internet]. 2007;12(4 (58)):329-338. Available from: https://sid.ir/paper/18544/en}

IEEE: Copy

P. MOHAMMADI TORBATI, and N. RASTEGAR RAD, “COMPARSION BETWEEN CATION-EXCHANGE CHROMATOGRAPHY USING HBGOLD IVD AND REFERENCE METHOD OF MICRO-CHROMATOGRAPHY IN QUALIFICATIVE MEASUREMENT OF HEMOGLOBIN A_{2,” RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH), vol. 12, no. 4 (58), pp. 329–338, 2007, [Online]. Available: https://sid.ir/paper/18544/en}

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