MOLECULAR ANALYSIS OF VIRULENCE GENES STX1, STX2 IN ESCHERICHIA COLI ISOLATED FROM STOOL SAMPLES AND DETERMINING OF THEIR ANTIBIOTIC RESISTANCE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

RAMEZANI U.; PARVIZ M.; KHALAJZADEH S.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of North Khorasan University of Medical Sciences Year:2016 | Volume:8 | Issue:1 Page(s): 47-58

Download Full-Text

Persian Verion

View:

1,357

Download:

Cites:

Information Journal Paper

Title

MOLECULAR ANALYSIS OF VIRULENCE GENES STX1, STX2 IN ESCHERICHIA COLI ISOLATED FROM STOOL SAMPLES AND DETERMINING OF THEIR ANTIBIOTIC RESISTANCE

Author(s)

RAMEZANI U. | PARVIZ M. | KHALAJZADEH S. | Issue Writer Certificate

Keywords

ESCHERICHIA COLIQ2

STECQ1

VIRULENCE GENESQ1

Abstract

Background and Objectives: Pathotypes of ESCHERICHIA COLI, including EHEC (Enterohemorragic ESCHERICHIA COLI) are an important cause of enteric disease and extra-intestinal. Shiga toxin E.coli (STEC), along with EHEC strains are known as the most common cause of diarrhea in developing countries. The purpose of this study was to evaluate the VIRULENCE GENES in E.coli strain by Multiplex-PCR method isolated from diarrhea and determine antibiotic resistance.Materials and Methods: In this cross-sectional study, after collecting 100 samples of human diarrhea of outpatient treatment centers in Tehran, culture and biochemical tests and 55 strains of ESCHERICHIA COLI strains isolated and at the same time was extracted DNA. Antimicrobial susceptibility testing was performed by disk diffusion and E-test method based on CLSI guidelines with antibiotics from various groups. Statistical data were analyzed with SPSS version 19, using descriptive statistics.Results: Most E.coli isolates resistant to antibiotics Erythromycin (100%) and Ampicillin (94%) and were sensitive to Nitrofurantoin (96%) Amikacin (100%). The test E-Test greatest Resistance to antibiotics erythromycin (100%) and the most sensitivity was observed to antibiotics Gentamycin and Amikacin (100%). According to the results of molecular analysis of 55 human samples, was identified in 12 samples (21.8%) Stx2 gene and 4 sample (7.2%) Stx1 gene.Conclusion: The results obtained in this study compared to other studies showed that involved age, sample type, geographic location, type of diet, personal hygiene, seasons, identification and isolation procedures.

Cites

No record.

References

Cite

APA: Copy

RAMEZANI, U., PARVIZ, M., & KHALAJZADEH, S.. (2016). MOLECULAR ANALYSIS OF VIRULENCE GENES STX1, STX2 IN ESCHERICHIA COLI ISOLATED FROM STOOL SAMPLES AND DETERMINING OF THEIR ANTIBIOTIC RESISTANCE. JOURNAL OF NORTH KHORASAN UNIVERSITY OF MEDICAL SCIENCES, 8(1), 47-58. SID. https://sid.ir/paper/187182/en

Vancouver: Copy

RAMEZANI U., PARVIZ M., KHALAJZADEH S.. MOLECULAR ANALYSIS OF VIRULENCE GENES STX1, STX2 IN ESCHERICHIA COLI ISOLATED FROM STOOL SAMPLES AND DETERMINING OF THEIR ANTIBIOTIC RESISTANCE. JOURNAL OF NORTH KHORASAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2016;8(1):47-58. Available from: https://sid.ir/paper/187182/en

IEEE: Copy

U. RAMEZANI, M. PARVIZ, and S. KHALAJZADEH, “MOLECULAR ANALYSIS OF VIRULENCE GENES STX1, STX2 IN ESCHERICHIA COLI ISOLATED FROM STOOL SAMPLES AND DETERMINING OF THEIR ANTIBIOTIC RESISTANCE,” JOURNAL OF NORTH KHORASAN UNIVERSITY OF MEDICAL SCIENCES, vol. 8, no. 1, pp. 47–58, 2016, [Online]. Available: https://sid.ir/paper/187182/en

Related Journal Papers