NGF GENE TRANSFER TO BONE MARROW MESENCHYMAL STROMAL CELLS USING FIV AND HIV BASED LENTIVIRAL VECTORS

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AKBARI S.; PARVANEH TAFRESHI A.; ABBASI SH.; MOMEN H.; MASOUMI M.

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Journal: JOURNAL OF CELL & TISSUE Year:2012 | Volume:3 | Issue:3 Page(s): 211-220

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Title

NGF GENE TRANSFER TO BONE MARROW MESENCHYMAL STROMAL CELLS USING FIV AND HIV BASED LENTIVIRAL VECTORS

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Keywords

FELINE IMMUNODEFICIENCY VIRUS LENTIVIRAL VECTORQ2

HUMAN IMMUNODEFICIENCY VIRUS LENTIVIRAL VECTORQ2

BONE MARROW STROMAL CELLSQ2

NGFQ2

Abstract

Aim: In this study, gene transfer efficiency of human lentiviral vector (HIV) into rat BONE MARROW STROMAL CELLS was compared with that of the feline lentiviral vector (FIV).Material and Methods: To produce lentiviral vectors, a triple transfection with a shuttle vector, a backbone which carries either the GFP gene as a control or the NGF gene, and an envelope vector was performed using calcium-phosphate method in HEK-293T cell line. Supernatants of HEK-293T cell line containing the produced viruses were then exposed to BONE MARROW STROMAL CELLS (BMSCs) of the rat.Results: While BONE MARROW STROMAL CELLS infected with HIV-NGF showed a relatively high immunoreactivity of NGF, those infected with FIV-NGF did not express NGF. Furthermore, BMSCs infected with FIV-NGF viruses containing antibiotic resistant gene could not survive in the culture media containing the antibiotic.Conclusion: FIV based lentiviral vectors may not have a desirable efficacy for gene transfer into BONE MARROW STROMAL CELLS.

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APA: Copy

AKBARI, S., PARVANEH TAFRESHI, A., ABBASI, SH., MOMEN, H., & MASOUMI, M.. (2012). NGF GENE TRANSFER TO BONE MARROW MESENCHYMAL STROMAL CELLS USING FIV AND HIV BASED LENTIVIRAL VECTORS. JOURNAL OF CELL & TISSUE, 3(3), 211-220. SID. https://sid.ir/paper/189118/en

Vancouver: Copy

AKBARI S., PARVANEH TAFRESHI A., ABBASI SH., MOMEN H., MASOUMI M.. NGF GENE TRANSFER TO BONE MARROW MESENCHYMAL STROMAL CELLS USING FIV AND HIV BASED LENTIVIRAL VECTORS. JOURNAL OF CELL & TISSUE[Internet]. 2012;3(3):211-220. Available from: https://sid.ir/paper/189118/en

IEEE: Copy

S. AKBARI, A. PARVANEH TAFRESHI, SH. ABBASI, H. MOMEN, and M. MASOUMI, “NGF GENE TRANSFER TO BONE MARROW MESENCHYMAL STROMAL CELLS USING FIV AND HIV BASED LENTIVIRAL VECTORS,” JOURNAL OF CELL & TISSUE, vol. 3, no. 3, pp. 211–220, 2012, [Online]. Available: https://sid.ir/paper/189118/en

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