DESIGNING AND CONSTRUCTING THE LENTIVIRAL VECTOR CODING MIRNA-499A AND TRANSDUCTION OF HUMAN MESENCHYMAL STEM CELLS

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NESHATI VAJIHEH; Mollazadeh Samaneh; FAZLY BAZZAZ BIBI SEDIGHEH; MOJARRAD MAJID; NESHATI ZEINAB; KERACHIAN MOHAMMAD AMIN

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Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2018 | Volume:28 | Issue:166 Page(s): 21-29

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Title

DESIGNING AND CONSTRUCTING THE LENTIVIRAL VECTOR CODING MIRNA-499A AND TRANSDUCTION OF HUMAN MESENCHYMAL STEM CELLS

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Keywords

LENTIVIRAL VECTORSQ1

MICRORNAQ2

MIRNA-499A-3P/5PQ2

DIFFERENTIATIONQ2

Abstract

Background and purpose: LENTIVIRAL VECTORS (LVs) are suitable candidates for gene delivery to cells with stable and high-level of transgene expression in target cells. MICRORNAs (miRNAs, miRs) are non-protein coding, short (~22 nucleotides) and single-stranded RNAs that act as post-transcriptional regulators of gene expression, and are involved in various cellular processes, including proliferation, DIFFERENTIATION and apoptosis. Several studies have shown that miR-499a promotes cardiac DIFFERENTIATION in cardiac stem cells. So, the aim of our study was to construct lentiviruses carrying miRNA-499a. Materials and methods: Specific sequences of miRNA-499a (3p and 5p) were designed and constructed. Then, miRNA-499a was cloned into lentiviral vector. Analytical digestion and nucleotide sequence analysis were performed to ensure successful introduction of the miRNA to the vector. Then, the lentiviral particles produced (miRNA-499a-3p and miRNA-499a-5p) were used for transduction of human bone marrow-derived mesenchymal stem cells (hBM-MSCs). Results: Analytical digestion and sequence analysis confirmed the accuracy of the constructs. The high expression of eGFP represented the high efficiency of transfection and transduction. The lentiviral particles carrying MIRNA-499A-3P/5P were made and could transduce hBM-MSCs. Conclusion: In this study we made lentiviral particles carrying miR-499a that could be used for DIFFERENTIATION of stem cells to cardiomyocytes.

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APA: Copy

NESHATI, VAJIHEH, Mollazadeh, Samaneh, FAZLY BAZZAZ, BIBI SEDIGHEH, MOJARRAD, MAJID, NESHATI, ZEINAB, & KERACHIAN, MOHAMMAD AMIN. (2018). DESIGNING AND CONSTRUCTING THE LENTIVIRAL VECTOR CODING MIRNA-499A AND TRANSDUCTION OF HUMAN MESENCHYMAL STEM CELLS. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 28(166 ), 21-29. SID. https://sid.ir/paper/44563/en

Vancouver: Copy

NESHATI VAJIHEH, Mollazadeh Samaneh, FAZLY BAZZAZ BIBI SEDIGHEH, MOJARRAD MAJID, NESHATI ZEINAB, KERACHIAN MOHAMMAD AMIN. DESIGNING AND CONSTRUCTING THE LENTIVIRAL VECTOR CODING MIRNA-499A AND TRANSDUCTION OF HUMAN MESENCHYMAL STEM CELLS. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2018;28(166 ):21-29. Available from: https://sid.ir/paper/44563/en

IEEE: Copy

VAJIHEH NESHATI, Samaneh Mollazadeh, BIBI SEDIGHEH FAZLY BAZZAZ, MAJID MOJARRAD, ZEINAB NESHATI, and MOHAMMAD AMIN KERACHIAN, “DESIGNING AND CONSTRUCTING THE LENTIVIRAL VECTOR CODING MIRNA-499A AND TRANSDUCTION OF HUMAN MESENCHYMAL STEM CELLS,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 28, no. 166 , pp. 21–29, 2018, [Online]. Available: https://sid.ir/paper/44563/en

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