INVESTIGATION OF THE EFFECT OF INHALATION OF MAGNETIC IRON OXIDE NANOPARTICLES ON THE EXPRESSION OF P53 IN THE TESTIS TISSUE OF BALB/C MICE BY IMMUNOHISTOCHEMICALLY STAINING

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NASIRI Z.; MOSHTAGHIAN S.J.; ESMAEILI F.

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Journal: JOURNAL OF CELL & TISSUE Year:2017 | Volume:8 | Issue:1 Page(s): 22-31

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Title

INVESTIGATION OF THE EFFECT OF INHALATION OF MAGNETIC IRON OXIDE NANOPARTICLES ON THE EXPRESSION OF P53 IN THE TESTIS TISSUE OF BALB/C MICE BY IMMUNOHISTOCHEMICALLY STAINING

Author(s)

NASIRI Z. | MOSHTAGHIAN S.J. | ESMAEILI F. | Issue Writer Certificate

Keywords

EMBRYONIC CARCINOMA STEM CELLQ1

P19 CELLSQ1

NEURAL DIFFERENTIATIONQ1

CELL TRANSPLANTATIONQ1

CHICK EMBRYOQ2

Abstract

Aim: The present study was designed to investigate the effects of chitosan/poly vinyl alcohol scaffold incorporated by NRBE (neonatal rat brain extract) on neuronal differentiation of P19 EC (embryonal carcinoma) stem cells.Material and Methods: In order to induce neuronal phenotype, P19-derived EMBRYONIC CARCINOMA STEM CELLs were cultured on a rat chitosan/poly (vinyl alcohol) scaffold with newborn rat brain extracts. To evaluate the survival potential, migration and differentiation of the three-dimensional culture cells, these cells were grafted to the developing central nervous system of the CHICK EMBRYO. Finally, the transplanted cells were detected using specific staining and immunofluorescence methods.Results: Cresyl-Violet specific staining confirmed the neuronal phenotype of the transplantation cells. Also, the expression of specific neural protein, synaptophysin, was shown using the immunofluorescence process.Conclusion: The results of this study showed that P19 EMBRYONIC CARCINOMA STEM CELLs can be used as a source of pluripotent cells in transplantation studies in order to investigate cellular and molecular aspects of developmental and cellular differentiation.

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APA: Copy

NASIRI, Z., MOSHTAGHIAN, S.J., & ESMAEILI, F.. (2017). INVESTIGATION OF THE EFFECT OF INHALATION OF MAGNETIC IRON OXIDE NANOPARTICLES ON THE EXPRESSION OF P53 IN THE TESTIS TISSUE OF BALB/C MICE BY IMMUNOHISTOCHEMICALLY STAINING. JOURNAL OF CELL & TISSUE, 8(1 ), 22-31. SID. https://sid.ir/paper/189126/en

Vancouver: Copy

NASIRI Z., MOSHTAGHIAN S.J., ESMAEILI F.. INVESTIGATION OF THE EFFECT OF INHALATION OF MAGNETIC IRON OXIDE NANOPARTICLES ON THE EXPRESSION OF P53 IN THE TESTIS TISSUE OF BALB/C MICE BY IMMUNOHISTOCHEMICALLY STAINING. JOURNAL OF CELL & TISSUE[Internet]. 2017;8(1 ):22-31. Available from: https://sid.ir/paper/189126/en

IEEE: Copy

Z. NASIRI, S.J. MOSHTAGHIAN, and F. ESMAEILI, “INVESTIGATION OF THE EFFECT OF INHALATION OF MAGNETIC IRON OXIDE NANOPARTICLES ON THE EXPRESSION OF P53 IN THE TESTIS TISSUE OF BALB/C MICE BY IMMUNOHISTOCHEMICALLY STAINING,” JOURNAL OF CELL & TISSUE, vol. 8, no. 1 , pp. 22–31, 2017, [Online]. Available: https://sid.ir/paper/189126/en

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