ISOLATION OF VI ANTIGEN FROM SALMONELLA TYPHI TY2 AND ITS ANIMAL TEST FOR PREPARATION OF VACCINE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOGHBELI M.; SHAEBANI A.A.; AKBARIFAR E.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF MICROBIAL WORLD Year:2009 | Volume:2 | Issue:1 Page(s): 7-11

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

2,451

Download:

Cites:

Information Journal Paper

Title

ISOLATION OF VI ANTIGEN FROM SALMONELLA TYPHI TY2 AND ITS ANIMAL TEST FOR PREPARATION OF VACCINE

Author(s)

MOGHBELI M. | SHAEBANI A.A. | AKBARIFAR E. | Issue Writer Certificate

Keywords

SALMONELLA TYPHI TY2Q3

TYPHOIDQ3

VI ANTIGENQ1

VACCINEQ1

Abstract

Background and objective: TYPHOID is a worldwide disease. Salmonella Typhi is the most common agent causing TYPHOID. It is difficult to treat TYPHOID due to antibiotics multiple resistance and the bacterium is menace for future epidemic. Vaccination is the most successful method against these diseases. The aim of this study is the isolation of VI ANTIGEN from SALMONELLA TYPHI TY2 and evaluation of its immunogenicity in animal model.Material and methods: In this research, SALMONELLA TYPHI TY2 was cultured in Muller Hinton Agar Medium. After harvesting the cells and their suspention into physiologic serum, polysaccharide capsule was extracted by precipitation with cetavlone. Amount of Nucleic acid in the sample was tested with Spectrophotometere at 260 nm and amount of protein were investigated with Bradford method and SDS-PAGE and the Immunodiffusion method was used to study of isolated VI ANTIGEN. In final, immunogenesity of VI ANTIGEN was tested in mouse.Result: Amount of Nucleic acid and protein in the isolated sample were 0.08 mg/ml and 2.59mg respectively. In the SDS-PAGE, smear band of the Standard Vi was similar to isolated sample completely. The precipitation band in the Immunodiffusion method confirmed the presence of VI ANTIGEN. In the study of VI ANTIGEN on the mouse, 80 percent immunity was created. Also injection in Rabbit showed that LPS level in the sample does not cause much fever.Conclusion: With regard to the fact that the human VACCINE of TYPHOID is not produced in Iran at present, this method can be used to isolate VI ANTIGEN and this method can be introduced as a VACCINE candidate.

Cites

No record.

References

No record.

Cite

APA: Copy

MOGHBELI, M., SHAEBANI, A.A., & AKBARIFAR, E.. (2009). ISOLATION OF VI ANTIGEN FROM SALMONELLA TYPHI TY2 AND ITS ANIMAL TEST FOR PREPARATION OF VACCINE. JOURNAL OF MICROBIAL WORLD, 2(1), 7-11. SID. https://sid.ir/paper/189156/en

Vancouver: Copy

MOGHBELI M., SHAEBANI A.A., AKBARIFAR E.. ISOLATION OF VI ANTIGEN FROM SALMONELLA TYPHI TY2 AND ITS ANIMAL TEST FOR PREPARATION OF VACCINE. JOURNAL OF MICROBIAL WORLD[Internet]. 2009;2(1):7-11. Available from: https://sid.ir/paper/189156/en

IEEE: Copy

M. MOGHBELI, A.A. SHAEBANI, and E. AKBARIFAR, “ISOLATION OF VI ANTIGEN FROM SALMONELLA TYPHI TY2 AND ITS ANIMAL TEST FOR PREPARATION OF VACCINE,” JOURNAL OF MICROBIAL WORLD, vol. 2, no. 1, pp. 7–11, 2009, [Online]. Available: https://sid.ir/paper/189156/en

Related Journal Papers