CONTAMINATION OF FRESH BEEF TO SALMONELLA TYPHIMURIUM AND SALMONELLA ENTERITIDIS IN SANANDAJ DURING 2012

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KARIMI DAREHABI H.; ESMAILNESHAD F.; EBRAHIMI MOHAMMADI K.

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Journal: JOURNAL OF FOOD HYGIENE Year:2012 | Volume:2 | Issue:3 (7) Page(s): 41-48

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Title

CONTAMINATION OF FRESH BEEF TO SALMONELLA TYPHIMURIUM AND SALMONELLA ENTERITIDIS IN SANANDAJ DURING 2012

Author(s)

KARIMI DAREHABI H. | ESMAILNESHAD F. | EBRAHIMI MOHAMMADI K. | Issue Writer Certificate

Keywords

BEEFQ2

SALMONELLA TYPHIMURIUMQ2

SALMONELLA ENTERITIDISQ2

MULTIPLEX PCRQ2

Abstract

Salmonella infection is among the main food-borne gastrointestinal disease. Meat has been recognized as a major source of human illness caused by Salmonella serovars. The presence of Salmonella was detected in 60 samples of fresh BEEF from retailsof Sanandaj. The presence ofSalmonella was assessed by conventional culture method and confirmed by PCR assay. To confirm the identification of isolated colonies as Salmonella spp. and determining serovars as typhimurium and enteritidis serovars, a MULTIPLEX PCR assay, using three pairs of primers were employed. S141 and S139 for Inv Agene, specific for the genus of Salmonella, Fli15 and Tym for FliCgene, specific for typhimurium serovar and Prot6e-5 and Prot6e-6 forProt6E gene, specific for Enteritidis serovar.12 samples 20% were determined as contaminated with Salmonella spp with microbial culture method but with PCR method only seven samples 11.66% were confirmed.4 samples (6.6%) of isolated colonies were confirmed as SALMONELLA TYPHIMURIUM and any number of isolated colonies were confirmed as SALMONELLA ENTERITIDIS, the other isolated colonis were belong to other salmonella serovars. This study showed a relatively highprevalence of salmonella in fresh BEEF from Sanandaj.

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APA: Copy

KARIMI DAREHABI, H., ESMAILNESHAD, F., & EBRAHIMI MOHAMMADI, K.. (2012). CONTAMINATION OF FRESH BEEF TO SALMONELLA TYPHIMURIUM AND SALMONELLA ENTERITIDIS IN SANANDAJ DURING 2012. JOURNAL OF FOOD HYGIENE, 2(3 (7)), 41-48. SID. https://sid.ir/paper/222955/en

Vancouver: Copy

KARIMI DAREHABI H., ESMAILNESHAD F., EBRAHIMI MOHAMMADI K.. CONTAMINATION OF FRESH BEEF TO SALMONELLA TYPHIMURIUM AND SALMONELLA ENTERITIDIS IN SANANDAJ DURING 2012. JOURNAL OF FOOD HYGIENE[Internet]. 2012;2(3 (7)):41-48. Available from: https://sid.ir/paper/222955/en

IEEE: Copy

H. KARIMI DAREHABI, F. ESMAILNESHAD, and K. EBRAHIMI MOHAMMADI, “CONTAMINATION OF FRESH BEEF TO SALMONELLA TYPHIMURIUM AND SALMONELLA ENTERITIDIS IN SANANDAJ DURING 2012,” JOURNAL OF FOOD HYGIENE, vol. 2, no. 3 (7), pp. 41–48, 2012, [Online]. Available: https://sid.ir/paper/222955/en

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