ASSIMILATION OF BIOSENSOR BY MEANS OF MICROCONTACT SCHEME BASED ON MOLECULARLY IMPRINTED POLYMER FOR DETECTION OF SALMONELLA TYPHI

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ANVAR S.A.A.; RAZAVILAR V.; AKBARI ADERGANI B.; MOTALEBI MOGHANJOUGHI A.A.; AHARI H.

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Journal: Journal of Food Technology and Nutrition Year:2016 | Volume:13 | Issue:3 (51) Page(s): 73-86

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Title

ASSIMILATION OF BIOSENSOR BY MEANS OF MICROCONTACT SCHEME BASED ON MOLECULARLY IMPRINTED POLYMER FOR DETECTION OF SALMONELLA TYPHI

Author(s)

Keywords

ASSIMILATIONQ2

BIOSENSORQ2

MICROCONTACTQ2

SALMONELLA TYPHIQ2

Abstract

Introduction: The detection of the bacteria mass in food products using traditional methods is a long process that might take four to ten days. The advances in nano technology has made the design of selective and intelligent sensors possible that are capable of precisely detecting the bacteria mass in a short time.Materials and Methods: In this research, the MIP (molecularly imprinted polymer) was utilized. First, in order to prepare the molecular imprints and produce the polymer, metaacrylic acid (MAA) monomers were used. Then, covalence bonds between the MAA monomers created a white polymer and hydrogen bonds between the antibody and MAA were formed. By using fluorescence converter and its connection to the antibody of SALMONELLA TYPHI bacteria, it is possible to detect the SALMONELLA TYPHI anti-gene. The florescence disseminations after neighbouring were quantified by a spectrofluorometer.Results: The results indicated that, it is possible to detect the concentration of SALMONELLA TYPHI bacteria with a minimum mass of 10 Mol in polluted waters. Moreover, the designed sensor was evaluated in terms of configuration specificity. Also, the Ecoli bacteria were inseminated in an aquatic environment containing SALMONELLA TYPHI proving that there was no interference with the sensor function. Besides, sensor sensitivity was examined up to 60 days and showed that sensor performance could be verified up to 52 days and then starts to diminish.Conclusion: The applications of BIOSENSORs to detect microbial contaminations have been increased due to the shortening of detection time while retaining the required characteristics and sensitivity.

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تشخیص اگزوتوکسین باکتری استافیلوکوکوس ارئوس توسط روش پتانسیومتری در مقایسه با طیف سنجی مبتنی بر نانوذرات اصلاح شده (متصل به پادتن)

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APA: Copy

ANVAR, S.A.A., RAZAVILAR, V., AKBARI ADERGANI, B., MOTALEBI MOGHANJOUGHI, A.A., & AHARI, H.. (2016). ASSIMILATION OF BIOSENSOR BY MEANS OF MICROCONTACT SCHEME BASED ON MOLECULARLY IMPRINTED POLYMER FOR DETECTION OF SALMONELLA TYPHI. JOURNAL OF FOOD TECHNOLOGY AND NUTRITION, 13(3 (51)), 73-86. SID. https://sid.ir/paper/143327/en

Vancouver: Copy

ANVAR S.A.A., RAZAVILAR V., AKBARI ADERGANI B., MOTALEBI MOGHANJOUGHI A.A., AHARI H.. ASSIMILATION OF BIOSENSOR BY MEANS OF MICROCONTACT SCHEME BASED ON MOLECULARLY IMPRINTED POLYMER FOR DETECTION OF SALMONELLA TYPHI. JOURNAL OF FOOD TECHNOLOGY AND NUTRITION[Internet]. 2016;13(3 (51)):73-86. Available from: https://sid.ir/paper/143327/en

IEEE: Copy

S.A.A. ANVAR, V. RAZAVILAR, B. AKBARI ADERGANI, A.A. MOTALEBI MOGHANJOUGHI, and H. AHARI, “ASSIMILATION OF BIOSENSOR BY MEANS OF MICROCONTACT SCHEME BASED ON MOLECULARLY IMPRINTED POLYMER FOR DETECTION OF SALMONELLA TYPHI,” JOURNAL OF FOOD TECHNOLOGY AND NUTRITION, vol. 13, no. 3 (51), pp. 73–86, 2016, [Online]. Available: https://sid.ir/paper/143327/en

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