ASSESSMENT OF THE SURVIVAL RATE OF NON-CULTIVABLE FORMS OF HELICOBACTER PYLORI IN WATER SAMPLES

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Chamanrokh Parastoo; SHAHHOSSEINY MOHAMMAD HASSAN; MAZAHERI ASSADI MAHNAZ; NEJADSATTARI TAHER; ESMAILI DAVOOD

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Journal: JOURNAL OF MICROBIAL WORLD Year:2017 | Volume:9 | Issue:4 (29) Page(s): 337-345

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Title

ASSESSMENT OF THE SURVIVAL RATE OF NON-CULTIVABLE FORMS OF HELICOBACTER PYLORI IN WATER SAMPLES

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Keywords

HELICOBACTER PYLORIQ2

COCCOIDQ1

GLMM GENEQ2

PCRQ2

CULTUREQ2

Abstract

H. Pyloriis an important cause of human peptic ulcers and stomach cancers. Once the bacterium is placed in the water, it changes into a viable, but non-cultivable COCCOID form, which is considered as an important factor to spread out the infection. The aim of this study was to evaluate the survival rate of COCCOID forms of H. pylori in water samples, using PCR and CULTURE methods. This experimental study was performed on 10 strains of H. pylori isolated from clinical specimens. Isolates were added to water at the temperatures of 4oC, 22oC, and 37oC and incubated at intervals of 1 and 2 months. Each time, the samples were CULTUREd on Brucella blood Agar medium. Following DNA extraction, the presence of GLMM GENE was confirmed using PCR method. Furthermore, the sensitivity and specificity of PCR method were evaluated. While culturing in first month at 4oC showed no H. pylori COCCOID growth on medium, some positive results (growth rate of 10% and 20%, respectively) were detected at 22oC and 37oC during the same month. No positive result was obtained during the second month. Performing PCR, with more sensitivity as compared to culturing method, identified H. pylori COCCOID growth of 10%, 30%, and 40%, for the first month, and 0%, 20%, and 30% for the second month at 4°C, 22°C, and 37°C, respectively. The results of this study showed that the non-cultivable cocoid forms of H. pylori in water can be detected by non-culturing methods such as PCR which is sensitive, specific, and accurate.

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APA: Copy

Chamanrokh, Parastoo, SHAHHOSSEINY, MOHAMMAD HASSAN, MAZAHERI ASSADI, MAHNAZ, NEJADSATTARI, TAHER, & ESMAILI, DAVOOD. (2017). ASSESSMENT OF THE SURVIVAL RATE OF NON-CULTIVABLE FORMS OF HELICOBACTER PYLORI IN WATER SAMPLES. JOURNAL OF MICROBIAL WORLD, 9(4 (29)), 337-345. SID. https://sid.ir/paper/189226/en

Vancouver: Copy

Chamanrokh Parastoo, SHAHHOSSEINY MOHAMMAD HASSAN, MAZAHERI ASSADI MAHNAZ, NEJADSATTARI TAHER, ESMAILI DAVOOD. ASSESSMENT OF THE SURVIVAL RATE OF NON-CULTIVABLE FORMS OF HELICOBACTER PYLORI IN WATER SAMPLES. JOURNAL OF MICROBIAL WORLD[Internet]. 2017;9(4 (29)):337-345. Available from: https://sid.ir/paper/189226/en

IEEE: Copy

Parastoo Chamanrokh, MOHAMMAD HASSAN SHAHHOSSEINY, MAHNAZ MAZAHERI ASSADI, TAHER NEJADSATTARI, and DAVOOD ESMAILI, “ASSESSMENT OF THE SURVIVAL RATE OF NON-CULTIVABLE FORMS OF HELICOBACTER PYLORI IN WATER SAMPLES,” JOURNAL OF MICROBIAL WORLD, vol. 9, no. 4 (29), pp. 337–345, 2017, [Online]. Available: https://sid.ir/paper/189226/en

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