ISOLATION OF BACILLUS CEREUS FROM FOODS AND STUDYING THE CYTOTOXICITY OF THEM ON VERO CELLS

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DEILAMI KHIABANI Z.; NASIRI SEMNANI SH.

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Journal: JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES) Year:2016 | Volume:9 | Issue:1 (32) Page(s): 69-77

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Title

ISOLATION OF BACILLUS CEREUS FROM FOODS AND STUDYING THE CYTOTOXICITY OF THEM ON VERO CELLS

Author(s)

DEILAMI KHIABANI Z. | NASIRI SEMNANI SH. | Issue Writer Certificate

Keywords

BACILLUC CEREUSQ2

NHEQ2

HBLQ2

PCRQ2

VERO CELLSQ2

Abstract

Introduction & Objective: Bacillus cereus is an important enter toxigenic food borne pathogen. It contains NHE and HBL complex enterotoxins. The genes of these complex can be used for characterization of enterotoxin production in B. cereus. The objective of this study is to detect enter toxigenic B. cereus and assessing the incidence of it in foods.Material and Methods: 220 food samples including; 80 meat products, 20 dairy, 20 rice and 100 salad samples, have been collected in the years 2012-2014. Using biochemical and molecular tests, the B. cereus positive food samples has been detected. B.cereus isolates checked for NHE and HBL complex by specific primers. B. cereus isolates which contain both NHE and HBL have been used for cytotoxic assay on VERO CELLS.Results: Among 100 B.cereus isolates, 40 contain NHE, 23 contain HBL and 17 isolates contain both complexes. The recent isolates have been used for cytotoxic assay on VERO CELLS.Conclusion: PCR is a prompt, reliable method for detection of enter toxigenic bacteria. Enhancing awareness about virulence and prevalence of genes involved in food poisoning would be effective in prevention of food poisoning.

Cites

Bacillus cereus Assessment in Dried Vegetables Distributed in Tehran, Iran

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APA: Copy

DEILAMI KHIABANI, Z., & NASIRI SEMNANI, SH.. (2016). ISOLATION OF BACILLUS CEREUS FROM FOODS AND STUDYING THE CYTOTOXICITY OF THEM ON VERO CELLS. JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES), 9(1 (32)), 69-77. SID. https://sid.ir/paper/191626/en

Vancouver: Copy

DEILAMI KHIABANI Z., NASIRI SEMNANI SH.. ISOLATION OF BACILLUS CEREUS FROM FOODS AND STUDYING THE CYTOTOXICITY OF THEM ON VERO CELLS. JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES)[Internet]. 2016;9(1 (32)):69-77. Available from: https://sid.ir/paper/191626/en

IEEE: Copy

Z. DEILAMI KHIABANI, and SH. NASIRI SEMNANI, “ISOLATION OF BACILLUS CEREUS FROM FOODS AND STUDYING THE CYTOTOXICITY OF THEM ON VERO CELLS,” JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES), vol. 9, no. 1 (32), pp. 69–77, 2016, [Online]. Available: https://sid.ir/paper/191626/en

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