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Information Journal Paper

Title

THE SURVEY ON TYPE AND CONCENTRATION OF SOME HORMONIC TREATMENT ON TISSUE CULTURE OF THE ENDANGERED SPECIES RECH. ASTRAGALUS FRIDAE. F

Pages

  25-34

Abstract

 The goal of this experiment has been to study the impact of the type and concentration of some growth tuners on the organogenesis of a type of Astragalus, ASTRAGALUS FRIDAE. The impact of four kinds of hormones including Naftalineacetic acid (NAA), Dechlorophonoxyacetic acid-2, 4 (D-2, 4), N6 (2-isopentyl) adenine (2ip) and 6-Benzylaminopourine (BAP) on the culture of various parts of the plant, Astragus fridae were studied. The explants were selected from the apical meristem, the stem, the leaf and the root of the sterile plantlets. The hormones were used in the form of 2IP/NAA and BAP/2, 4-D and sixteen types of treatments were applied for every combination. In the culture environment using different concentrations of the hormone BAP/2, 4-D the root explants produced CALLUSes at a quantity and volume rate less than other explants and without any differentiation and organogenesis, but the other explants including the apical meristem, stem and leaf explants produced aerial organs and roots using different hormonic concentrations and combinations in both MS culture environments. The experiments and analyze of the data resulted determined that the meristem explants are the best explants in regeneration of the complete plant in the in vitro condition and the hormonic combination 2IP/NAA with different concentrations reveals better CALLUS and organogenesis.At the final stage, the plants produced on culturing stage transferred to the pots containing sterile peat & perlit and were neutralized by the Hoagland liquid and after a week, these plants transferred to the natural soil gathered from their original ecosystem.

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    Cite

    APA: Copy

    ARBABIAN, S., & MOGHANLOO, M.. (2009). THE SURVEY ON TYPE AND CONCENTRATION OF SOME HORMONIC TREATMENT ON TISSUE CULTURE OF THE ENDANGERED SPECIES RECH. ASTRAGALUS FRIDAE. F. JOURNAL OF DEVELOPMENTAL BIOLOGY, 1(2), 25-34. SID. https://sid.ir/paper/193089/en

    Vancouver: Copy

    ARBABIAN S., MOGHANLOO M.. THE SURVEY ON TYPE AND CONCENTRATION OF SOME HORMONIC TREATMENT ON TISSUE CULTURE OF THE ENDANGERED SPECIES RECH. ASTRAGALUS FRIDAE. F. JOURNAL OF DEVELOPMENTAL BIOLOGY[Internet]. 2009;1(2):25-34. Available from: https://sid.ir/paper/193089/en

    IEEE: Copy

    S. ARBABIAN, and M. MOGHANLOO, “THE SURVEY ON TYPE AND CONCENTRATION OF SOME HORMONIC TREATMENT ON TISSUE CULTURE OF THE ENDANGERED SPECIES RECH. ASTRAGALUS FRIDAE. F,” JOURNAL OF DEVELOPMENTAL BIOLOGY, vol. 1, no. 2, pp. 25–34, 2009, [Online]. Available: https://sid.ir/paper/193089/en

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