THE RATE OF HEP-2 CELLULAR INVASION OF SALMONELLASEROGROUPS IN DIARRHEA PATIENTS

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SOLTAN DALLAL M.M.; RAHIMI FORUSHANI A.; SHARIFI YAZDI K.; RASTEGAR LARI A.; NIKMANESH B.; AMIN HARATI F.

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Journal: MEDICAL LABORATORY JOURNAL Year:2013 | Volume:7 | Issue:1 Page(s): 17-21

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Title

THE RATE OF HEP-2 CELLULAR INVASION OF SALMONELLASEROGROUPS IN DIARRHEA PATIENTS

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Keywords

SALMONELLAQ3

DIARRHEAQ3

CELL INVASIONQ2

CELL CULTUREQ3

Abstract

Background and Objective: Gastroenteritis is one of the most common forms of Salmonellosis, which is a worldwide problem. The invasive characteristic of intestinal bacteria is one of their pathogenicity mechanisms, which can be easily investigated by CELL CULTURE technique. In this study, the invasive characteristic of some SALMONELLA serogroup was investigated by usingHEP-2 cell.Material and Methods: Therectalsoaps were prepared from 280 DIARRHEA patients referred to Imam Khomeyni and children medical centres, 140 with bloody DIARRHEA and 140 with watery DIARRHEA as a comparison group. The rectal soaps were obtained from the patients before taking any antibiotics, and 140 rectal specimens were taken from healthy people as a control group. All the samples were inoculated in differential and selective media, like Hektoen and Xylose lysine deoxycholate (XLD) agar. After incubation at 37C for 24 hours, the colonies were examined and identified by conventional biochemical and serological tests. Using HEP-2, cellular invasion characteristic of SALMONELLA serogroups was assessed. Moreover, the antibiotic resistance patterns were performed according to clinical and laboratory standards institute (CLSI).Results: Of all tested samples, 35 (8.3%) are SALMONELLA strains. The frequency of SALMONELLA is reported for bloody DIARRHEA (5.2%), watery DIARRHEA (1.7%) and control group (1.4%).The most abundant serogroups with invasive characteristic, using HEP-2 CELL CULTURE, areserogroup B (62.9%) and D (17.2%).Conclusion: The results obtained in this study show that the majority of SALMONELLA isolates are without invasive characteristic.

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APA: Copy

SOLTAN DALLAL, M.M., RAHIMI FORUSHANI, A., SHARIFI YAZDI, K., RASTEGAR LARI, A., NIKMANESH, B., & AMIN HARATI, F.. (2013). THE RATE OF HEP-2 CELLULAR INVASION OF SALMONELLASEROGROUPS IN DIARRHEA PATIENTS. MEDICAL LABORATORY JOURNAL, 7(1), 17-21. SID. https://sid.ir/paper/194733/en

Vancouver: Copy

SOLTAN DALLAL M.M., RAHIMI FORUSHANI A., SHARIFI YAZDI K., RASTEGAR LARI A., NIKMANESH B., AMIN HARATI F.. THE RATE OF HEP-2 CELLULAR INVASION OF SALMONELLASEROGROUPS IN DIARRHEA PATIENTS. MEDICAL LABORATORY JOURNAL[Internet]. 2013;7(1):17-21. Available from: https://sid.ir/paper/194733/en

IEEE: Copy

M.M. SOLTAN DALLAL, A. RAHIMI FORUSHANI, K. SHARIFI YAZDI, A. RASTEGAR LARI, B. NIKMANESH, and F. AMIN HARATI, “THE RATE OF HEP-2 CELLULAR INVASION OF SALMONELLASEROGROUPS IN DIARRHEA PATIENTS,” MEDICAL LABORATORY JOURNAL, vol. 7, no. 1, pp. 17–21, 2013, [Online]. Available: https://sid.ir/paper/194733/en

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