STUDY OF DIENTAMOEBA FRAGILIS BY IRON HEMATOXYLIN STAINING AND NESTED-PCR METHODS IN CHALOUS HEALTHCARE REFERS

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ALIZADEH SHARGH SH.; GHAZANCHAEI A.; AYETOLLAHI A.A.; KHANDAN DEL A.; POURASGHARI B.; ESTAKHRI R.

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Journal: MEDICAL LABORATORY JOURNAL Year:2011 | Volume:4 | Issue:2 Page(s): 1-6

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Title

STUDY OF DIENTAMOEBA FRAGILIS BY IRON HEMATOXYLIN STAINING AND NESTED-PCR METHODS IN CHALOUS HEALTHCARE REFERS

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Keywords

DIENTAMOEBAQ3

POLYMERASE CHAIN REACTIONQ3

HEMATOXYLINQ2

CHALOUS REGIONQ3

Abstract

Bachground and objectives: DIENTAMOEBA fragilis is a habitant protozoon in human colon causing clinical symptoms, such as local stomach pain, weight loss, lack of appetite and flatulence. It is important to diagnose this infection correctly and differentiate it from other Protozoa. In this study PCR and Iron HEMATOXYLIN methods were used to detection of this protozoon in Chalous Medical centers refers in 2010.Material and Methods: The stool samples (n=302) of this cross-sectional study were selected via cluster random sampling. After wet mount study the samples were preserved in PVA (for staining) and Ethanol (for molecular(. The samples were studied both Staining and Molecular methods. Sensitivity and specificity were assessed.Results: Of 302 samples, six of them are positive via staining method (1.99%) and five by molecular method. All negative results with staining method are also negative with PCR. Contamination with E.coli in 2 samples and with Balstocystis homonis were seen in one sample. Sensitivity and specificity of PCR was 85% and 100% respectively.Conclusion: The discrepancy between two methods maybe caused by observer errors in staining method and unsynchronized molecular and microscopic studies.

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APA: Copy

ALIZADEH SHARGH, SH., GHAZANCHAEI, A., AYETOLLAHI, A.A., KHANDAN DEL, A., POURASGHARI, B., & ESTAKHRI, R.. (2011). STUDY OF DIENTAMOEBA FRAGILIS BY IRON HEMATOXYLIN STAINING AND NESTED-PCR METHODS IN CHALOUS HEALTHCARE REFERS. MEDICAL LABORATORY JOURNAL, 4(2), 1-6. SID. https://sid.ir/paper/194753/en

Vancouver: Copy

ALIZADEH SHARGH SH., GHAZANCHAEI A., AYETOLLAHI A.A., KHANDAN DEL A., POURASGHARI B., ESTAKHRI R.. STUDY OF DIENTAMOEBA FRAGILIS BY IRON HEMATOXYLIN STAINING AND NESTED-PCR METHODS IN CHALOUS HEALTHCARE REFERS. MEDICAL LABORATORY JOURNAL[Internet]. 2011;4(2):1-6. Available from: https://sid.ir/paper/194753/en

IEEE: Copy

SH. ALIZADEH SHARGH, A. GHAZANCHAEI, A.A. AYETOLLAHI, A. KHANDAN DEL, B. POURASGHARI, and R. ESTAKHRI, “STUDY OF DIENTAMOEBA FRAGILIS BY IRON HEMATOXYLIN STAINING AND NESTED-PCR METHODS IN CHALOUS HEALTHCARE REFERS,” MEDICAL LABORATORY JOURNAL, vol. 4, no. 2, pp. 1–6, 2011, [Online]. Available: https://sid.ir/paper/194753/en

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