CLONING AND EXPRESSION OF NANOBODY GENE AGAINST ENTEROTOXIN B OF STAPHYLOCOCCUS AUREUS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

TAVASSOLI Z.; ROUHANI NEJAD H.; FALLAH MEHRABADI J.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF ADVANCED BIOMEDICAL SCIENCES Year:2017 | Volume:6 | Issue:4 Page(s): 496-503

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

674

Download:

Cites:

Information Journal Paper

Title

CLONING AND EXPRESSION OF NANOBODY GENE AGAINST ENTEROTOXIN B OF STAPHYLOCOCCUS AUREUS

Author(s)

TAVASSOLI Z. | ROUHANI NEJAD H. | FALLAH MEHRABADI J. | Issue Writer Certificate

Keywords

NANOBODYQ2

VHHQ1

ENTROTOXIN BQ2

STAPHYLOCOCCUS AUREUSQ1

Abstract

Background & Objectives: STAPHYLOCOCCUS AUREUS bacteria causes many different diseases by secretion of various enterotoxins. Therefore, it is necessary to develop ways that facilitate the detection of enterotoxins. Nowadays, immunochemical methods which are based on monoclonal antibody technology are used. The heavy chain antibodies that are called VHH or Nano body were found in blood serum of the Camelidae family. The unique properties of this antibody such as their binding to small molecules like toxins make them attractive candidates for the development of immunodiagnostic tests. The present study was done to achieve a VHH molecules against Staphylococcus enterotoxin B.Materials & Methods: Freighting phage library for isolate private Nano bodies against enterotoxin B was done in previous works. Next, pCANTAB 5E vector that consists VHH, extracted from E.coli bacteria strain xl1blue, and after doing PCR process with relative primers, sub cloning in pET21a (+) as an expression vector with cut sites NdeI and XhoI was done. Transformation in E.coli bacteria strain BL21 (DE3) was done. Then, the cells effected with IPTG and producing time, and other terms were optimized. Finally, the expression of the protein with SDS-PAGE and western blot techniques was evaluated.Result: For proving cloning of nano body gene in pET21a (+) vector, nucleotide sequence of gene was analyzed, and transforming to E.coli bacteria strain BL21 (DE3) was successful. After inspiration, active protein in cell was seen by SDS-PAGE technique and proved by western blot.Conclusion: cloning, sub cloning, and nonabody expression were surveyed in this research. Production of this protein can help to develop new therapeutic methods and produce vaccine against enterotoxin B of STAPHYLOCOCCUS AUREUS.

Cites

No record.

References

No record.

Cite

APA: Copy

TAVASSOLI, Z., ROUHANI NEJAD, H., & FALLAH MEHRABADI, J.. (2017). CLONING AND EXPRESSION OF NANOBODY GENE AGAINST ENTEROTOXIN B OF STAPHYLOCOCCUS AUREUS. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, 6(4), 496-503. SID. https://sid.ir/paper/203844/en

Vancouver: Copy

TAVASSOLI Z., ROUHANI NEJAD H., FALLAH MEHRABADI J.. CLONING AND EXPRESSION OF NANOBODY GENE AGAINST ENTEROTOXIN B OF STAPHYLOCOCCUS AUREUS. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES[Internet]. 2017;6(4):496-503. Available from: https://sid.ir/paper/203844/en

IEEE: Copy

Z. TAVASSOLI, H. ROUHANI NEJAD, and J. FALLAH MEHRABADI, “CLONING AND EXPRESSION OF NANOBODY GENE AGAINST ENTEROTOXIN B OF STAPHYLOCOCCUS AUREUS,” JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, vol. 6, no. 4, pp. 496–503, 2017, [Online]. Available: https://sid.ir/paper/203844/en

Related Journal Papers