ASSESSMENT OF VIRULENCE FACTORS(ELASTASE AND PHOSPHOLIPASE) ANDANTIBIOTIC SUSCEPTIBILITY IN PSEUDOMONAS AERUGINOSA ISOLATED FROMCLINICAL SAMPLES

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RAHBAR ZARE MAHYA; NOORBAKHSH FATEMEH; Honarmand Jahromi Sahar

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2018 | Volume:8 | Issue:29 Page(s): 31-38

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Information Journal Paper

Title

ASSESSMENT OF VIRULENCE FACTORS(ELASTASE AND PHOSPHOLIPASE) ANDANTIBIOTIC SUSCEPTIBILITY IN PSEUDOMONAS AERUGINOSA ISOLATED FROMCLINICAL SAMPLES

Author(s)

RAHBAR ZARE MAHYA | NOORBAKHSH FATEMEH | Honarmand Jahromi Sahar | Issue Writer Certificate

Keywords

PSEUDOMONAS AERUGINOSAQ2

PLCHQ1

LASBQ1

PLCNQ1

Abstract

Aim and Background: PSEUDOMONAS AERUGINOSA is one of the most important pathogen causeinfection in hospital in world. Different virulence gene exsist in this bacteria, which interfere inpathogenesis and infection. The aim of this study was evaluation of antibiotic susceptibility pattern and identify virulencegenes PLCH, LASB, PLCN of P. aeruginosa isolated from clinical samples. Material and methods: In this study 100 PSEUDOMONAS AERUGINOSA isolated of human cases ofburn in 1395. Antibiotic susceptibility test was performed by disk diffusion method. Genomic DNA wereextracted from isolated strains and Multiplex-PCR performed to determine the preavalence ofeach of the tested genes. Result: The scope age of patients were 20-90 years, and 46 patient were male and 52 werefemale. The prevalence of PLCN and PLCH observed 98 percent and LASB 100 percent. Thehighest resistance to antibiotics were observed in ampicillin and cefoxitin with the frequency of93% and 96% respectively. Conclusion: The high prevalence of PLCH, LASB, PLCN gene in PSEUDOMONAS AERUGINOSA isolatedof skin case of burn revealed that this genes are an important virulence factors in burn infections.

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APA: Copy

RAHBAR ZARE, MAHYA, NOORBAKHSH, FATEMEH, & Honarmand Jahromi, Sahar. (2018). ASSESSMENT OF VIRULENCE FACTORS(ELASTASE AND PHOSPHOLIPASE) ANDANTIBIOTIC SUSCEPTIBILITY IN PSEUDOMONAS AERUGINOSA ISOLATED FROMCLINICAL SAMPLES. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 8(29 ), 31-38. SID. https://sid.ir/paper/204418/en

Vancouver: Copy

RAHBAR ZARE MAHYA, NOORBAKHSH FATEMEH, Honarmand Jahromi Sahar. ASSESSMENT OF VIRULENCE FACTORS(ELASTASE AND PHOSPHOLIPASE) ANDANTIBIOTIC SUSCEPTIBILITY IN PSEUDOMONAS AERUGINOSA ISOLATED FROMCLINICAL SAMPLES. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2018;8(29 ):31-38. Available from: https://sid.ir/paper/204418/en

IEEE: Copy

MAHYA RAHBAR ZARE, FATEMEH NOORBAKHSH, and Sahar Honarmand Jahromi, “ASSESSMENT OF VIRULENCE FACTORS(ELASTASE AND PHOSPHOLIPASE) ANDANTIBIOTIC SUSCEPTIBILITY IN PSEUDOMONAS AERUGINOSA ISOLATED FROMCLINICAL SAMPLES,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 8, no. 29 , pp. 31–38, 2018, [Online]. Available: https://sid.ir/paper/204418/en

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