REPLACING OF ANTIBIOTIC WITH NANO SILVER IN BULL SPERM EXTENDERS

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HAYATI ROODBARI NASIM; AMIRNIA SIROUS; PARIVAR KAZEM; SEYEDALIAN REYHANEH

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2013 | Volume:3 | Issue:10 Page(s): 59-65

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Title

REPLACING OF ANTIBIOTIC WITH NANO SILVER IN BULL SPERM EXTENDERS

Author(s)

HAYATI ROODBARI NASIM | AMIRNIA SIROUS | PARIVAR KAZEM | SEYEDALIAN REYHANEH | Issue Writer Certificate

Keywords

NANO SILVER PARTICLESQ2

SEMEN EXTENDERQ2

BULL SPERMQ3

ANTIBIOTICSQ2

Abstract

Aim and Background: Silver nano particles (NPs) or nano silver (NS) are clusters of silver atoms that range in diameter from 1 to 100nm and are attracting interest as antibacterial and antimicrobial agents of applications in medicine. Resistance to antimicrobial agents by pathogenic bacteria has emerged in recent years and is a major reason for replacing of ANTIBIOTICS with nano silver, but yet nanoparticle hazardous and toxic effects have not been studied extensively.Materials and Methods: One of the agent of SEMEN EXTENDERs is ANTIBIOTICS. We replaced ANTIBIOTICS with nano silver and investigated mobility, progressive of sperm. Also result of nano silver on the growth of semen’s bacteria was studied and them was compared with penicillin and streptomycin in similar conditions.Results: Semen was diluted with citrate sodium, Yolk extender and was divided into nine portions. The control (sham) SEMEN EXTENDER solution contained extended semen and no antibiotic and nano silver, whereas streptomycin and penicillin solutions contained extended semen plus 1.0 mg/mL of streptomycin and penicillin. Also semen was diluted with Citrate sodium, Yolk extender supplemented with different concentrations of silver nano particle. Here, the effects of different concentration of silver NPs on BULL SPERM at frequent times after1, 3 hours was evaluated. Therefore, the Mean percentages of motile and progressively motile sperm did not differ significantly among control (sham) and antibiotic and nano silver -containing solutions after 1 hour. but after 3 hour, nano silver was cytotoxic on BULL SPERM cells in more than 0.1mL concentration .(p<0.001).Conclusion: Control extended semen samples from ejaculates of bulls were contaminated with gram pasitive bacteria. Samples of antibiotic and concentration of 0.005mL, 0.01mL nano silver had growth a few of bacteria. Nano silver has good antibacterial properties in SEMEN EXTENDER in more than 0.05mL concentration. Thus, It can be suggested that ANTIBIOTICS may be replaced with nano silver as antimicrobial agent in SEMEN EXTENDERs in Some few concentration.

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APA: Copy

HAYATI ROODBARI, NASIM, AMIRNIA, SIROUS, PARIVAR, KAZEM, & SEYEDALIAN, REYHANEH. (2013). REPLACING OF ANTIBIOTIC WITH NANO SILVER IN BULL SPERM EXTENDERS. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 3(10), 59-65. SID. https://sid.ir/paper/204470/en

Vancouver: Copy

HAYATI ROODBARI NASIM, AMIRNIA SIROUS, PARIVAR KAZEM, SEYEDALIAN REYHANEH. REPLACING OF ANTIBIOTIC WITH NANO SILVER IN BULL SPERM EXTENDERS. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2013;3(10):59-65. Available from: https://sid.ir/paper/204470/en

IEEE: Copy

NASIM HAYATI ROODBARI, SIROUS AMIRNIA, KAZEM PARIVAR, and REYHANEH SEYEDALIAN, “REPLACING OF ANTIBIOTIC WITH NANO SILVER IN BULL SPERM EXTENDERS,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 3, no. 10, pp. 59–65, 2013, [Online]. Available: https://sid.ir/paper/204470/en

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