Identification of extermophilies producing l-glutaminase and l-asparaginase from Larijanhotspring

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NOORMOHAMADI ELHAM; FARAHMAND MAHNAZ; ESMAEILI RASTAGHI AHMAD REZA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2018 | Volume:8 | Issue:31 Page(s): 29-38

Download Full-Text

Persian Verion

View:

1,502

Download:

Cites:

Information Journal Paper

Title

Identification of extermophilies producing l-glutaminase and l-asparaginase from Larijanhotspring

Author(s)

NOORMOHAMADI ELHAM | FARAHMAND MAHNAZ | ESMAEILI RASTAGHI AHMAD REZA | Issue Writer Certificate

Keywords

Thermophilic bacteriaQ1

L-asparaginaseQ2

L-glutaminaseQ2

therapeutic agentQ1

Abstract

Background and Objective: The most frequently used sources of more stable enzymes are Thermophilic bacteria. In recent years microbial L-asparaginase has been widely used as therapeutic agent in the treatment of human cancers. Microbial L-glutaminase is currently used in the treatment of acute lymphoblastic leukemia (ALL) and human immunodeficiency virus (HIV) because of its potential applications in medicine. The aim of present study was to screen new potent Thermophilic bacterial isolates producing L-asparaginase and L-glutaminase. Methods: Larijan hotspring was chosen as the location for isolation of the Thermophilic bacteria. Culture and morphological and biochemical tests, were performed. All the bacterial isolates were screened in ortherto L-asparaginase and L-glutaminase enzyme activity using Rapid Plate assay method. M9 modified media incorporated L-asparaginase or L-glutaminase and phenol red as indicator were used. Thermophilic strains were later identified by 16SrRNA gene ribotyping followed by PCR. Results: A total of six isolates recovered from Larijan hotspring were found to belong to Bacillus licheniformis. The L-asparaginase activity was observed in LT-1, LT-2, LT-3, LT-4, LT-5 strains. The L-glutaminase produced by Bacillus licheniformis LT-1, LT-3, LT-5 and LT-6 strains. Three thermophilic LT-1, LT-3, LT-5 strains with L-glutaminase and L-asparaginase activity have been successfully isolated. Conclusion: Larijan hot spring is one of the potential sources of useful microbial products that can be used as a source of useful different biological products like enzymes.

Cites

No record.

References

No record.

Cite

APA: Copy

NOORMOHAMADI, ELHAM, FARAHMAND, MAHNAZ, & ESMAEILI RASTAGHI, AHMAD REZA. (2018). Identification of extermophilies producing l-glutaminase and l-asparaginase from Larijanhotspring. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 8(31 ), 29-38. SID. https://sid.ir/paper/204703/en

Vancouver: Copy

NOORMOHAMADI ELHAM, FARAHMAND MAHNAZ, ESMAEILI RASTAGHI AHMAD REZA. Identification of extermophilies producing l-glutaminase and l-asparaginase from Larijanhotspring. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2018;8(31 ):29-38. Available from: https://sid.ir/paper/204703/en

IEEE: Copy

ELHAM NOORMOHAMADI, MAHNAZ FARAHMAND, and AHMAD REZA ESMAEILI RASTAGHI, “Identification of extermophilies producing l-glutaminase and l-asparaginase from Larijanhotspring,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 8, no. 31 , pp. 29–38, 2018, [Online]. Available: https://sid.ir/paper/204703/en

Related Journal Papers