ISOLATION AND IDENTIFICATION OF NATIVE IRANIAN L-ASPARAGINASE PRODUCING BACTERIA

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YAZDANI ROYA; MOBINI DEHKORDI MOHSEN; RASTEGARI ALI ASGHAR

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Journal: JOURNAL OF MICROBIAL WORLD Year:2012 | Volume:5 | Issue:1-2 (12) Page(s): 39-46

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Title

ISOLATION AND IDENTIFICATION OF NATIVE IRANIAN L-ASPARAGINASE PRODUCING BACTERIA

Author(s)

YAZDANI ROYA | MOBINI DEHKORDI MOHSEN | RASTEGARI ALI ASGHAR | Issue Writer Certificate

Keywords

L-ASPARAGINASEQ2

ENZYME ACTIVITYQ2

SUBMERGED FERMENTATIONQ2

NESSLERIZATIONQ2

Abstract

Background and Objectives: L-ASPARAGINASE can be effectively used for the treatment of acute lymphoblastic leukemia and tumor cells as well as in food industries. The L-ASPARAGINASE is the first enzyme with antitumor activity that is intensively studied in different human cancers. Purpose of this study was isolation and identification of Iranian native L-ASPARAGINASE producing bacteria and determination of their enzyme activities. Material and Methods: The soil and water samples were collected from various cities of Iran. For screening of asparaginase producing bacteria, species were cultivated on M9 agar media containing phenol red as pH indicator. Enzyme production and assay were performed by SUBMERGED FERMENTATION and NESSLERIZATION method respectively. Results: 11out of 61 isolated species could produce the enzyme and one of them showed maximum enzyme productivity. This thermophilic species was belonged to Bacillus genus based on biochemical test results. This species could produce 1613.178 (U/ml) enzymes after 48 hours incubation in 45oC. Conclusion: Because of application of L-ASPARAGINASE in various fields, the enzyme produced by this species can be suitable for utilization after more investigations.

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APA: Copy

YAZDANI, ROYA, MOBINI DEHKORDI, MOHSEN, & RASTEGARI, ALI ASGHAR. (2012). ISOLATION AND IDENTIFICATION OF NATIVE IRANIAN L-ASPARAGINASE PRODUCING BACTERIA. JOURNAL OF MICROBIAL WORLD, 5(1-2 (12)), 39-46. SID. https://sid.ir/paper/189274/en

Vancouver: Copy

YAZDANI ROYA, MOBINI DEHKORDI MOHSEN, RASTEGARI ALI ASGHAR. ISOLATION AND IDENTIFICATION OF NATIVE IRANIAN L-ASPARAGINASE PRODUCING BACTERIA. JOURNAL OF MICROBIAL WORLD[Internet]. 2012;5(1-2 (12)):39-46. Available from: https://sid.ir/paper/189274/en

IEEE: Copy

ROYA YAZDANI, MOHSEN MOBINI DEHKORDI, and ALI ASGHAR RASTEGARI, “ISOLATION AND IDENTIFICATION OF NATIVE IRANIAN L-ASPARAGINASE PRODUCING BACTERIA,” JOURNAL OF MICROBIAL WORLD, vol. 5, no. 1-2 (12), pp. 39–46, 2012, [Online]. Available: https://sid.ir/paper/189274/en

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