Review and compare Mycobacterium paratuberculosis isolated from livestock in the provinces of Tehran and Alborz by PCR, SSR methods

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GHAFARI OMID; FOROOHI FATEMEH; Nemati Fahimeh

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Paper Information

Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2018 | Volume:8 | Issue:31 Page(s): 65-74

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Information Journal Paper

Title

Review and compare Mycobacterium paratuberculosis isolated from livestock in the provinces of Tehran and Alborz by PCR, SSR methods

Author(s)

GHAFARI OMID | FOROOHI FATEMEH | Nemati Fahimeh | Issue Writer Certificate

Keywords

Mycobacterium aviumQ1

ParatuberculosisQ1

SSR1and SSR2Q1

Abstract

Aim and Background: Mycobacterium avium subspecies Paratuberculosis (MAP) is causative agent of Johne's disease. The disease is worldwide spread and causes important economic losses in livestock industries. Materials and Methods: This study was performed on 30 bacterial isolates that different by geographical area (provinces of Tehran and Alborez) and host (cattle, sheep and goat). PCR method was used for amplification of two locos SSR1 and SSR2. Results: The results showed that isolates have different structurally based on SSR1and SSR2 each other and also with the standard strain. The locus of Insertion and Deletion due to repetitive sequences in tandem, different genetic patterns observed that the ability to distinguish between strains provides. Conclusion: Considering the two alleles of each gene, genetic samples were placed in two types.

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APA: Copy

GHAFARI, OMID, FOROOHI, FATEMEH, & Nemati, Fahimeh. (2018). Review and compare Mycobacterium paratuberculosis isolated from livestock in the provinces of Tehran and Alborz by PCR, SSR methods. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 8(31 ), 65-74. SID. https://sid.ir/paper/204711/en

Vancouver: Copy

GHAFARI OMID, FOROOHI FATEMEH, Nemati Fahimeh. Review and compare Mycobacterium paratuberculosis isolated from livestock in the provinces of Tehran and Alborz by PCR, SSR methods. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2018;8(31 ):65-74. Available from: https://sid.ir/paper/204711/en

IEEE: Copy

OMID GHAFARI, FATEMEH FOROOHI, and Fahimeh Nemati, “Review and compare Mycobacterium paratuberculosis isolated from livestock in the provinces of Tehran and Alborz by PCR, SSR methods,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 8, no. 31 , pp. 65–74, 2018, [Online]. Available: https://sid.ir/paper/204711/en

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