ASSESSMENT OF GENETIC RELATIONSHIPS CLONES OF IRANIAN AND FOREIGN SUGARCANE BY SSR AND RAPD MOLECULAR MARKERS

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KARAMI S.; BARAT SHOSHTARI M.; MALEKI ZANJANI B.; SHIRAN B.

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Paper Information

Journal: IRANIAN JOURNAL OF BIOLOGY Year:2010 | Volume:23 | Issue:3 Page(s): 353-366

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Information Journal Paper

Title

ASSESSMENT OF GENETIC RELATIONSHIPS CLONES OF IRANIAN AND FOREIGN SUGARCANE BY SSR AND RAPD MOLECULAR MARKERS

Author(s)

KARAMI S. | BARAT SHOSHTARI M. | MALEKI ZANJANI B. | SHIRAN B. | Issue Writer Certificate

Keywords

RAPDQ2

SSRQ2

GENETIC DIVERSITYQ2

SUGARCANEQ2

VARIANCE ANALYSISQ3

Abstract

This study aims to assessment of GENETIC DIVERSITY between Iranian SUGARCANE clones (released clones) and foreign cultivars and also identifies unknown accessions collected from Iran by 65 and 7 RAPD markers and SSR markers, respectively. Twenty-eight RAPD and seven SSR markers were polymorphic and produced a PCR product and number of fragments amplified by the RAPD and SSR polymorphic markers ranged from 6-27 (with an average of 15.1) and 5-14 (with an average of 9.33), respectively.Both molecular markers revealed a high degree of GENETIC DIVERSITY in SUGARCANE germplasm also; RAPD and SSR markers could separate identify accessions collected from Iran. For both markers a high similarity in dendrogram topologies was obtained although some differences were observed. All dendrograms, including that obtained by the combined use of both types of marker data, depicted relationships among the cultivars, depending upon their geographic region and/or pedigree information. The results of analysis of molecular variance (AMOVA) indicated that genetic differentiation among cultivars within each region was greater than between regions.We also used 6 sorghum and 1 maize SSR primers pair for exploring the cross transferability of sorghum and maize SSR markers to SUGARCANE but none of them, showed amplification products.

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