PGIP-PG INTERACTION, CLONING AND PARTIAL CHARACTERIZATION OF PGIP GENE FROM PHASEOLUS VULGARIS CV. DERAKHSHAN

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HOSSEINZADEH KOLAGAR A.; ZAMANI M.R.; MOTALEBI M.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2004 | Volume:17 | Issue:2 Page(s): 126-137

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Information Journal Paper

Title

PGIP-PG INTERACTION, CLONING AND PARTIAL CHARACTERIZATION OF PGIP GENE FROM PHASEOLUS VULGARIS CV. DERAKHSHAN

Author(s)

HOSSEINZADEH KOLAGAR A. | ZAMANI M.R. | MOTALEBI M. | Issue Writer Certificate

Keywords

PGIP-PG INTERACTION- PGIP GENEQ2

FUSARIUM OXYSPORUMQ4

PHASEOLUS VULGARISQ4

Abstract

The cell walls of some dicotyledonous plants (pear, tomato, apple etc.) and a few monocotyledonous plants contain a polygalacturonase-inhibiting protein (PGIP) that binds to and inhibits fungal endopolygalacturonase. PGIP has a great potential for limiting fungal colonization by acting as both inhibitors and regulators of fungal polygalacturonases activity and by favoring the release of oligogalacturonide (OG) fragments, which are elicitors of a variety of defense responses. In this study, inhibitory effect of PGIP extracted from PHASEOLUS VULGARIS cv. Derakhshan on polygalacturonase enzyme of FUSARIUM OXYSPORUM was analyzed. PG enzyme was isolated from highly virulent isolate of F. oxysporum (F15). The PG activity was inhibited by hypocotyle PGIP about 54.5%.For pgip gene amplification, genomic DNA from leaves were used. The expected PCR product (1047 bp) was amplified with specific primers, which contain appropriate site for cloning. The PCR product was confirmed by restriction digestion. Pgip gene (1029 bp) was subcloned into pUC18 vector. The sequence of the cloned gene was aligned with related DNA sequences in the GeneBank. The results showed 100% similarity of this sequence to pgip-l of PHASEOLUS VULGARIS cv. Saxa. There was differed in 26 nt from pgip-2 sequence from PHASEOLUS VULGARIS cv. Pinto.

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APA: Copy

HOSSEINZADEH KOLAGAR, A., ZAMANI, M.R., & MOTALEBI, M.. (2004). PGIP-PG INTERACTION, CLONING AND PARTIAL CHARACTERIZATION OF PGIP GENE FROM PHASEOLUS VULGARIS CV. DERAKHSHAN. IRANIAN JOURNAL OF BIOLOGY, 17(2), 126-137. SID. https://sid.ir/paper/21180/en

Vancouver: Copy

HOSSEINZADEH KOLAGAR A., ZAMANI M.R., MOTALEBI M.. PGIP-PG INTERACTION, CLONING AND PARTIAL CHARACTERIZATION OF PGIP GENE FROM PHASEOLUS VULGARIS CV. DERAKHSHAN. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2004;17(2):126-137. Available from: https://sid.ir/paper/21180/en

IEEE: Copy

A. HOSSEINZADEH KOLAGAR, M.R. ZAMANI, and M. MOTALEBI, “PGIP-PG INTERACTION, CLONING AND PARTIAL CHARACTERIZATION OF PGIP GENE FROM PHASEOLUS VULGARIS CV. DERAKHSHAN,” IRANIAN JOURNAL OF BIOLOGY, vol. 17, no. 2, pp. 126–137, 2004, [Online]. Available: https://sid.ir/paper/21180/en

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