Optimization of growth and catalase production in Kocuria sp. ASB 107 in economic medium

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ASGARANI E.; Godini E.; FOOLADI J.

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Journal: Journal of Applied Biology Year:2019 | Volume:32 | Issue:1 Page(s): 98-107

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Title

Optimization of growth and catalase production in Kocuria sp. ASB 107 in economic medium

Author(s)

ASGARANI E. | Godini E. | FOOLADI J. | Issue Writer Certificate

Keywords

CatalaseQ1

Kocuria ASB107Q2

MolassesQ1

OptimizationQ1

WheyQ1

Abstract

Catalase is an enzyme capable of catalyzing the alteration of H2O2 to O2 and H2O. It has acquired importance due to its application in the textile industries. In the textile industry, H2O2 is used as a bleach. This process is performed at high pH. Therefore, alkaline Catalase is very suitable for H2O2 reduction. Kocuria ASB107 has a lot of alkaline Catalase. In order to achieve the highest rate of bacterial growth and Catalase production, sugar cane Molasses, sugar beet Molasses and Whey were used as cheap carbon sources. Growth curves were plotted and at the late logarithmic phase, fermentation product was harvested. Catalase activity was measured spectrophotometrically by monitoring the decrease in absorbance at 240 nm affected by the decomposition of hydrogen peroxide. Bacterial growth was also estimated from the weight of dry biomass. The highest biomass (5. 19 g/L) and Catalase activity (2136. 25 U/mL) were found in medium consisted 1% Molasses and 2. 5 % yeast extract. In addition with the 4% Whey as a carbon source, Catalase activity and growth were 3032. 5 U/mL and 6. 16 g/L respectively. The results showed Molasses and Whey are suitable and inexpensive substrate. On the other hand inorganic nitrogen sources such as urea are not suitable for the production of Catalase and cell growth.

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APA: Copy

ASGARANI, E., Godini, E., & FOOLADI, J.. (2019). Optimization of growth and catalase production in Kocuria sp. ASB 107 in economic medium. JOURNAL OF APPLIED BIOLOGY (JOURNAL OF SCIENCE), 32(1 ), 98-107. SID. https://sid.ir/paper/22651/en

Vancouver: Copy

ASGARANI E., Godini E., FOOLADI J.. Optimization of growth and catalase production in Kocuria sp. ASB 107 in economic medium. JOURNAL OF APPLIED BIOLOGY (JOURNAL OF SCIENCE)[Internet]. 2019;32(1 ):98-107. Available from: https://sid.ir/paper/22651/en

IEEE: Copy

E. ASGARANI, E. Godini, and J. FOOLADI, “Optimization of growth and catalase production in Kocuria sp. ASB 107 in economic medium,” JOURNAL OF APPLIED BIOLOGY (JOURNAL OF SCIENCE), vol. 32, no. 1 , pp. 98–107, 2019, [Online]. Available: https://sid.ir/paper/22651/en

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