RECOMBINANT EXPRESSION OF DOMAIN III OF DENGUE VIRUS ENVELOPE PROTEIN (SEROTYPE-1)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

FAHIMI H.; SADEGHIZADEH M.; HEIDARI S.; MOHAMMADIPOUR M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Police Medicine Year:2012 | Volume:1 | Issue:1 (1) Page(s): 54-61

Download Full-Text

Persian Verion

View:

1,188

Download:

Cites:

Information Journal Paper

Title

RECOMBINANT EXPRESSION OF DOMAIN III OF DENGUE VIRUS ENVELOPE PROTEIN (SEROTYPE-1)

Author(s)

FAHIMI H. | SADEGHIZADEH M. | HEIDARI S. | MOHAMMADIPOUR M. | Issue Writer Certificate

Keywords

DENGUE VIRUSQ4

ENVELOPE PROTEINQ4

VACCINEQ4

Abstract

Aim: The aim of this study is to produce a recombinant antigen from the ENVELOPE PROTEIN of DENGUE VIRUS serotype 1, as a dengue VACCINE candidate. Therefore, we intend to study expression efficiency of domain III in a bacterial host.Materials and Methods: We used the following software: Megalign (sequence alignment); Modeller (protein structure prediction), and Optimizer (coding gene sequence optimization). The optimum coding sequence was synthesized and cloned in a pET21 expression vector. Stable protein expression and optimization of antigen production was performed in an Origami strain of E. coli.Results: A consensus sequence for the domain III of DENGUE VIRUS serotype 1 was produced by multiple alignment of sequences and named EDIII1. Next, we predicted EDIII1 properties by using bioinformatic software. To achieve a high expression level in the bacterial host, the coding sequence of this protein was optimized for appropriate CAI and GC content. The optimized gene was synthesized and cloned at theNde I and Xho I sites of the pET21 expression vector in an E. coli DH5α strain. In order to have efficient protein expression and the formation of disulfide bounds, we optimized expression of EDIII1 in the Origami strain. Protein purification, using Histag affinity chromatography columns was performed and the results identified by Western blot analysis.Conclusion: The results of this study showed the efficiency of a bacterial system to highly express domain III of the DENGUE VIRUS ENVELOPE PROTEIN.

Cites

No record.

References

No record.

Cite

APA: Copy

FAHIMI, H., SADEGHIZADEH, M., HEIDARI, S., & MOHAMMADIPOUR, M.. (2012). RECOMBINANT EXPRESSION OF DOMAIN III OF DENGUE VIRUS ENVELOPE PROTEIN (SEROTYPE-1). POLICE MEDICINE, 1(1 (1)), 54-61. SID. https://sid.ir/paper/227946/en

Vancouver: Copy

FAHIMI H., SADEGHIZADEH M., HEIDARI S., MOHAMMADIPOUR M.. RECOMBINANT EXPRESSION OF DOMAIN III OF DENGUE VIRUS ENVELOPE PROTEIN (SEROTYPE-1). POLICE MEDICINE[Internet]. 2012;1(1 (1)):54-61. Available from: https://sid.ir/paper/227946/en

IEEE: Copy

H. FAHIMI, M. SADEGHIZADEH, S. HEIDARI, and M. MOHAMMADIPOUR, “RECOMBINANT EXPRESSION OF DOMAIN III OF DENGUE VIRUS ENVELOPE PROTEIN (SEROTYPE-1),” POLICE MEDICINE, vol. 1, no. 1 (1), pp. 54–61, 2012, [Online]. Available: https://sid.ir/paper/227946/en

Related Journal Papers