RAPID DETECTION OF VIBRIO CHOLERAE USING HEXAPLEX PCR ASSAY

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ZEINODDINI MEHDI; SAEEDINIA ALI REZA; SADEGHI VAHID

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Paper Information

Journal: JOURNAL OF POLICE MEDICINE Year:2014 | Volume:3 | Issue:2 Page(s): 77-84

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Information Journal Paper

Title

RAPID DETECTION OF VIBRIO CHOLERAE USING HEXAPLEX PCR ASSAY

Author(s)

ZEINODDINI MEHDI | SAEEDINIA ALI REZA | SADEGHI VAHID | Issue Writer Certificate

Keywords

VIBRIO CHOLERAEQ3

PCRQ3

DETECTIONQ3

SPECIFICITYQ3

SENSITIVITYQ3

Abstract

Background: VIBRIO CHOLERAE is an important agent of diarrheal diseases in many parts of Asia and Africa. It is an enteric pathogen which produce a global pandemic of the disease. Rapid, sensitive and specific measurement of V. cholerae is an interest for many clinical laboratories. The aim of this study was to design and develop a hexa-plex PCR assay for rapid DETECTION of V. cholerae.Materials and Methods: Six pair of primers were designed for spe-cific amplification of the virulence and regulatory genes for VIBRIO CHOLERAE O1: cholera toxin enzymatic subunit A (ctxA) and B (ctxB), zonula occludens toxin (zot), accessory cholerae enterotoxin (ace), toxin- coregulated pilus (tcp) and outer membrane protein (ompW). Hexaplex PCR carried out using six primers and standard genes. Moreover, SENSITIVITY and SPECIFICITY of this method were determined.Results: Hexaplex PCR showed the presence of the virulence and regulatory genes for VIBRIO CHOLERAE O1 in the standard sample. In addition, SPECIFICITY was qualified and SENSITIVITY determined 100 cfu/ml in this method.Conclusion: Hexaplex PCR Assay could be used to design a kit for cholerae DETECTION.

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APA: Copy

ZEINODDINI, MEHDI, SAEEDINIA, ALI REZA, & SADEGHI, VAHID. (2014). RAPID DETECTION OF VIBRIO CHOLERAE USING HEXAPLEX PCR ASSAY. POLICE MEDICINE, 3(2), 77-84. SID. https://sid.ir/paper/227950/en

Vancouver: Copy

ZEINODDINI MEHDI, SAEEDINIA ALI REZA, SADEGHI VAHID. RAPID DETECTION OF VIBRIO CHOLERAE USING HEXAPLEX PCR ASSAY. POLICE MEDICINE[Internet]. 2014;3(2):77-84. Available from: https://sid.ir/paper/227950/en

IEEE: Copy

MEHDI ZEINODDINI, ALI REZA SAEEDINIA, and VAHID SADEGHI, “RAPID DETECTION OF VIBRIO CHOLERAE USING HEXAPLEX PCR ASSAY,” POLICE MEDICINE, vol. 3, no. 2, pp. 77–84, 2014, [Online]. Available: https://sid.ir/paper/227950/en

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