Determination of Benserazide Based on Bioluminescence Inhibition of Aequorin

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RAHMANI H.; Hasan sajedi R.

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Journal: JOURNAL OF BIOTECHNOLOGY Year:2019 | Volume:10 | Issue:4 Page(s): 627-633

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Information Journal Paper

Title

Determination of Benserazide Based on Bioluminescence Inhibition of Aequorin

Author(s)

RAHMANI H. | Hasan sajedi R. | Issue Writer Certificate

Keywords

AequorinQ1

BenserazideQ1

Bioluminescence inhibition assayQ1

Drug monitoringQ1

Abstract

Aims Aequorin as a bioluminescence protein due to ease of use, non-toxic, and high capability of detecting has long been the interest of researchers. The aim of this study was to design a method for accurate and simple detection of important therapeutic agents using a bioluminescence inhibition based assay by using Aequorin. Materials & Methods In this study, important drugs in therapeutic monitoring with structural similarity to Coelenterazine, were selected and their interaction with Aequorin was investigated. Further, the conditions of the bioluminescence assay were optimized to achieve the lowest detection limit. Findings Among the drugs whose effects have been tested on Aequorin, the only Benserazide resulted in inhibition of the bioluminescence activity. This analyte can significantly reduce the bioluminescence of Aequorin in a concentration-dependent manner. The best dose-response curve was obtained and IC50 of 0. 26μ M was calculated. The linear calibration curve was obtained in a range of about 100 to 1500nM with LOD and LOQ of 79 and 260nM, respectively. Furthermore, we demonstrated the application of the approach in human serum samples with a recovery of 97%. Guddem-Schild graph was plotted to determine the mechanism of inhibition which indicated that the IC50 of Benserazide changed in the presence of different concentrations of Coelenterazine. Conclusion The proposed method can be used for measuring Benserazide which can easily be applicable for real samples. Also, the results show that Benserazide inhibits the bioluminescence activity of Aequorin by competitive inhibition.

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APA: Copy

RAHMANI, H., & Hasan sajedi, R.. (2019). Determination of Benserazide Based on Bioluminescence Inhibition of Aequorin. JOURNAL OF BIOTECHNOLOGY, 10(4 ), 627-633. SID. https://sid.ir/paper/231257/en

Vancouver: Copy

RAHMANI H., Hasan sajedi R.. Determination of Benserazide Based on Bioluminescence Inhibition of Aequorin. JOURNAL OF BIOTECHNOLOGY[Internet]. 2019;10(4 ):627-633. Available from: https://sid.ir/paper/231257/en

IEEE: Copy

H. RAHMANI, and R. Hasan sajedi, “Determination of Benserazide Based on Bioluminescence Inhibition of Aequorin,” JOURNAL OF BIOTECHNOLOGY, vol. 10, no. 4 , pp. 627–633, 2019, [Online]. Available: https://sid.ir/paper/231257/en

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