APPLICATION OF ROTATABLE CENTRAL COMPOSITE DESIGN FOR OPTIMIZATION OF EXTRACELLULAR ALKALINE PROTEASE PRODUCTION FROM NEWLY ISOLATED BACILLUS PSEUDOFIRMUS MSB4

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

BORHANI MATIA SADAT; ETEMADIFAR ZAHRA; Jorjani Eisa

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Microbial Biology Year:2017 | Volume:5 | Issue:20 Page(s): 17-31

Download Full-Text

Persian Verion

View:

773

Download:

252

Cites:

Information Journal Paper

Title

APPLICATION OF ROTATABLE CENTRAL COMPOSITE DESIGN FOR OPTIMIZATION OF EXTRACELLULAR ALKALINE PROTEASE PRODUCTION FROM NEWLY ISOLATED BACILLUS PSEUDOFIRMUS MSB4

Author(s)

BORHANI MATIA SADAT | ETEMADIFAR ZAHRA | Jorjani Eisa | Issue Writer Certificate

Keywords

BACILLUS PSEUDOFIRMUS MSB4Q1

ALKALINE PROTEASEQ2

ONE-FACTOR-AT-A-TIME STRATEGYQ1

RESPONSE SURFACE METHODOLOGYQ1

ROTATABLE CENTRAL COMPOSITE DESIGNQ1

Abstract

Introduction: Microbial extracellular ALKALINE PROTEASEs are one of the most important industrial enzymes with a wide range of applications in various industries. In the present study, the production of an ALKALINE PROTEASE by BACILLUS PSEUDOFIRMUS MSB4 was evaluated using RESPONSE SURFACE METHODOLOGY (RSM).Materials and methods: Four significant independent variables were selected, based on the results of one-factor-at-a-time (OFAT) approach (xylose concentration, beef extract concentration, pH and temperature), for ROTATABLE CENTRAL COMPOSITE DESIGN (RCCD) to analyze the response pattern and to establish a model. The design consisted of total 30 runs, at five levels for each factor, with four replications of the center points. In addition, the presence of extracellular ALKALINE PROTEASE genes was evaluated by using PCR.Results: Maximum protease production (185.397 U/ml), 2.2 times higher than that of OFAT method and 3.7 times higher than the unoptimized conditions, was obtained by using 3% w/v beef extract, at pH 9 and 37oC according to the results of RCCD. Furthermore, the genes sub I, II and apr with the expected size (319, 486 and 194 bp, respectively) corresponding to the extracellular alkaline serine protease and metalloprotease were detected in MSB4 by using PCR.Discussion and conclusion: The experimental data well fitted the model (Adj R2: 0.9982) and the established quadratic model has a great ability to predict responses for new observations (Pred R2: 0.9967). The results showed that the OFAT and RSM strategies were a useful screening and optimization method for enhancing protease production of MSB4, respectively.

Cites

No record.

References

No record.

Cite

APA: Copy

BORHANI, MATIA SADAT, ETEMADIFAR, ZAHRA, & Jorjani, Eisa. (2017). APPLICATION OF ROTATABLE CENTRAL COMPOSITE DESIGN FOR OPTIMIZATION OF EXTRACELLULAR ALKALINE PROTEASE PRODUCTION FROM NEWLY ISOLATED BACILLUS PSEUDOFIRMUS MSB4. BIOLOGICAL JOURNAL OF MICROORGANISM, 5(20 ), 17-31. SID. https://sid.ir/paper/237344/en

Vancouver: Copy

BORHANI MATIA SADAT, ETEMADIFAR ZAHRA, Jorjani Eisa. APPLICATION OF ROTATABLE CENTRAL COMPOSITE DESIGN FOR OPTIMIZATION OF EXTRACELLULAR ALKALINE PROTEASE PRODUCTION FROM NEWLY ISOLATED BACILLUS PSEUDOFIRMUS MSB4. BIOLOGICAL JOURNAL OF MICROORGANISM[Internet]. 2017;5(20 ):17-31. Available from: https://sid.ir/paper/237344/en

IEEE: Copy

MATIA SADAT BORHANI, ZAHRA ETEMADIFAR, and Eisa Jorjani, “APPLICATION OF ROTATABLE CENTRAL COMPOSITE DESIGN FOR OPTIMIZATION OF EXTRACELLULAR ALKALINE PROTEASE PRODUCTION FROM NEWLY ISOLATED BACILLUS PSEUDOFIRMUS MSB4,” BIOLOGICAL JOURNAL OF MICROORGANISM, vol. 5, no. 20 , pp. 17–31, 2017, [Online]. Available: https://sid.ir/paper/237344/en

Related Journal Papers