INVESTIGATING THE L-ASPARAGINASE PRODUCTION IN THE YEAST YARROWIA ‎LIPOLYTICA

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DARVISHI FARSHAD; SHAMSI FERESHTEH

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Journal: BIOLOGICAL JOURNAL OF MICROORGANISM Year:2018 | Volume:78 | Issue:27 Page(s): 73-79

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Title

INVESTIGATING THE L-ASPARAGINASE PRODUCTION IN THE YEAST YARROWIA ‎LIPOLYTICA

Author(s)

DARVISHI FARSHAD | SHAMSI FERESHTEH | Issue Writer Certificate

Keywords

YARROWIA LIPOLYTICAQ1

ASPARAGINASEQ1

ANTICANCER DRUGQ1

PRODUCTIONQ2

Abstract

Introduction: L-Asparaginase catalyzes the hydrolysis of L-asparagine to L-aspartic acid and ammonia. This enzyme is used for the treatment of patients with acute lymphoblastic leukemia, melanosarcoma and lymphosarcoma. It is also used in the PRODUCTION of acrylamide-free foods. Considering the important applications of this enzyme, the aim of this study was to introduce a new microbial source for the PRODUCTION of eukaryotic L-asparaginase. Materials and Methods: The quality and quantity PRODUCTION of L-asparaginase was investigated in four strains of yeast YARROWIA LIPOLYTICA. The quality assessment of L-asparaginase PRODUCTION was done on asparagine minimal agar. Quantitative assay of the enzyme was done in Czapex Dox’s medium by spectrophotometric method using Nessler’s reagent. Results: All of four strains show that they are able to produce ASPARAGINASE making a purple halo around the colony. Y. lipolyticaDSM3286, DSM70562, DSM3218 and CBS6303 were produced 17.14, 11, 6.98 and 5.61 U/mL of ASPARAGINASE in Czapex Dox’s medium after 24 h, respectively. Discussion and Conclusion: Y. lipolytica DSM3286 was selected as the best ASPARAGINASE producer strain with the largest halo and the highest ASPARAGINASE PRODUCTION. The Y. lipolytica could be used as a potential source of L-asparaginase PRODUCTION.

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APA: Copy

DARVISHI, FARSHAD, & SHAMSI, FERESHTEH. (2018). INVESTIGATING THE L-ASPARAGINASE PRODUCTION IN THE YEAST YARROWIA ‎LIPOLYTICA. BIOLOGICAL JOURNAL OF MICROORGANISM, 78(27 ), 73-79. SID. https://sid.ir/paper/237403/en

Vancouver: Copy

DARVISHI FARSHAD, SHAMSI FERESHTEH. INVESTIGATING THE L-ASPARAGINASE PRODUCTION IN THE YEAST YARROWIA ‎LIPOLYTICA. BIOLOGICAL JOURNAL OF MICROORGANISM[Internet]. 2018;78(27 ):73-79. Available from: https://sid.ir/paper/237403/en

IEEE: Copy

FARSHAD DARVISHI, and FERESHTEH SHAMSI, “INVESTIGATING THE L-ASPARAGINASE PRODUCTION IN THE YEAST YARROWIA ‎LIPOLYTICA,” BIOLOGICAL JOURNAL OF MICROORGANISM, vol. 78, no. 27 , pp. 73–79, 2018, [Online]. Available: https://sid.ir/paper/237403/en

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