OPTIMIZATION OF RECOMBINANT LACCASE PRODUCTION BY YARROWIA LIPOLYTICA IN A MEDIUM CONTAINING GLUCOSE AS CARBON SOURCE WITH TAGUCHI METHOD

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DARVISHI FARSHAD; MORADI MARZIEH; Madzak Catherine; Jolivalt Claude

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Journal: Journal of Microbial Biology Year:2017 | Volume:6 | Issue:23 Page(s): 49-56

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Title

OPTIMIZATION OF RECOMBINANT LACCASE PRODUCTION BY YARROWIA LIPOLYTICA IN A MEDIUM CONTAINING GLUCOSE AS CARBON SOURCE WITH TAGUCHI METHOD

Author(s)

DARVISHI FARSHAD | MORADI MARZIEH | Madzak Catherine | Jolivalt Claude | Issue Writer Certificate

Keywords

YARROWIA LIPOLYTICAQ1

LACCASEQ2

OPTIMIZATIONQ2

TAGUCHI EXPERIMENTAL DESIGN METHODQ1

Abstract

Introduction: LACCASEs (EC 1.10.3.2; benzenediol: oxygen oxidoreductase) are copper-containing oxidases that use molecular oxygen to oxidize various aromatic and non-aromatic compounds. LACCASE is applied in delignification of lignocellulosic compounds for production of bioethanol, bioremediation of industrial wastewaters especially textile, food industries, and making biosensors.Materials and methods: The TAGUCHI EXPERIMENTAL DESIGN METHOD was used for OPTIMIZATION of LACCASE production in recombinant strain YARROWIA LIPOLYTICA YL4. A L-16 Taguchi orthogonal array was used to optimize the carbon and nitrogen sources along with vitamin in four levels.Results: The results showed that glucose, ammonium chloride, yeast extract and thiamine have significant effects on the production of LACCASE, respectively. The LACCASE activity reached to 1.52 U/mL after OPTIMIZATION of medium which is 7.6-fold higher than un-optimized medium.Discussion and conclusion: According to the analysis of results, the TAGUCHI EXPERIMENTAL DESIGN METHOD is a successful approach to increase LACCASE and recombinant proteins production in Y. lipolytica.

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APA: Copy

DARVISHI, FARSHAD, MORADI, MARZIEH, Madzak, Catherine, & Jolivalt, Claude. (2017). OPTIMIZATION OF RECOMBINANT LACCASE PRODUCTION BY YARROWIA LIPOLYTICA IN A MEDIUM CONTAINING GLUCOSE AS CARBON SOURCE WITH TAGUCHI METHOD. BIOLOGICAL JOURNAL OF MICROORGANISM, 6(23), 49-56. SID. https://sid.ir/paper/237443/en

Vancouver: Copy

DARVISHI FARSHAD, MORADI MARZIEH, Madzak Catherine, Jolivalt Claude. OPTIMIZATION OF RECOMBINANT LACCASE PRODUCTION BY YARROWIA LIPOLYTICA IN A MEDIUM CONTAINING GLUCOSE AS CARBON SOURCE WITH TAGUCHI METHOD. BIOLOGICAL JOURNAL OF MICROORGANISM[Internet]. 2017;6(23):49-56. Available from: https://sid.ir/paper/237443/en

IEEE: Copy

FARSHAD DARVISHI, MARZIEH MORADI, Catherine Madzak, and Claude Jolivalt, “OPTIMIZATION OF RECOMBINANT LACCASE PRODUCTION BY YARROWIA LIPOLYTICA IN A MEDIUM CONTAINING GLUCOSE AS CARBON SOURCE WITH TAGUCHI METHOD,” BIOLOGICAL JOURNAL OF MICROORGANISM, vol. 6, no. 23, pp. 49–56, 2017, [Online]. Available: https://sid.ir/paper/237443/en

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