Polymorphism Investigation of Tyrosinase Gene in Golden Rainbow Trout (Oncorhynchus mykiss)

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AKBARNEJAD B.; YOUSEFI SIAHKALROODI S.; ZARE KARIZI SH.

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Journal: Journal of Aquaculture Development Year:2017 | Volume:11 | Issue:1 Page(s): 1-7

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Information Journal Paper

Title

Polymorphism Investigation of Tyrosinase Gene in Golden Rainbow Trout (Oncorhynchus mykiss)

Author(s)

AKBARNEJAD B. | YOUSEFI SIAHKALROODI S. | ZARE KARIZI SH. | Issue Writer Certificate

Keywords

PolymorphismQ1

Golden Rainbow trout (Oncorhynchus mykiss)Q1

TyrosinaseQ1

PCR-RFLPQ1

Abstract

In order to identify Polymorphisms in the Tyrosinase gene for Golden rainbow trout (Oncorhynchus mykiss), a total of 100 different samples (fry) were prepared and DNA extraction was performed from their caudal fins. The polymerase chain reaction (PCR) was amplified fragment of 423bp the Tyrosinase gene. The amplified fragments were using specific primers (Tyr1. 2r and Tyr1. 2f) and Hinf1 cutting enzyme. In enzymatic digestion of the PCR products was observed three restricted patterns in 222bp and 201bp with NN (26 samples), and 423bp with MM (36 samples). The number of alleles N and M were 90 and 110 respectively, the gene frequency for allele N and M were. 45 and. 55 respectively. The number of genotypic frequencies NN, MN and MM were. 26, . 38 and. 36 respectively. The results showed that deviations from Hardy-Weinberg equilibrium were in all cases, due to artificial reproduction, probably.

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References

INVESTIGATION ON POLYMORPHISM OF MITOCHONDRIAL GENOME IN COBIA FISH RACHYCENTRON CANADUM IN THE NORTHERN WATERS OF THE PERSIAN GULF AND OMAN SEA

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APA: Copy

AKBARNEJAD, B., YOUSEFI SIAHKALROODI, S., & ZARE KARIZI, SH.. (2017). Polymorphism Investigation of Tyrosinase Gene in Golden Rainbow Trout (Oncorhynchus mykiss). JOURNAL OF AQUACULTURE DEVELOPMENT, 11(1 ), 1-7. SID. https://sid.ir/paper/242053/en

Vancouver: Copy

AKBARNEJAD B., YOUSEFI SIAHKALROODI S., ZARE KARIZI SH.. Polymorphism Investigation of Tyrosinase Gene in Golden Rainbow Trout (Oncorhynchus mykiss). JOURNAL OF AQUACULTURE DEVELOPMENT[Internet]. 2017;11(1 ):1-7. Available from: https://sid.ir/paper/242053/en

IEEE: Copy

B. AKBARNEJAD, S. YOUSEFI SIAHKALROODI, and SH. ZARE KARIZI, “Polymorphism Investigation of Tyrosinase Gene in Golden Rainbow Trout (Oncorhynchus mykiss),” JOURNAL OF AQUACULTURE DEVELOPMENT, vol. 11, no. 1 , pp. 1–7, 2017, [Online]. Available: https://sid.ir/paper/242053/en

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