INVESTIGATION ON POLYMORPHISM OF MITOCHONDRIAL GENOME IN COBIA FISH RACHYCENTRON CANADUM IN THE NORTHERN WATERS OF THE PERSIAN GULF AND OMAN SEA

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TALA MARYAM; KAZEMI DAMANE BAHRAM; LALOIE FARAMARZ; SOLTANI MEHDI; AZAD MANSOOR; KOOCHAKI AMENEH

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Journal: Researcher Bulletin of Medical Sciences Year:2012 | Volume:16 | Issue:5 (83) Page(s): 246-251

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Title

INVESTIGATION ON POLYMORPHISM OF MITOCHONDRIAL GENOME IN COBIA FISH RACHYCENTRON CANADUM IN THE NORTHERN WATERS OF THE PERSIAN GULF AND OMAN SEA

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Keywords

COBIAQ2

RACHYCENTRON CANADUMQ2

POLYMERASE CHAIN REACTIONQ3

POLYMORPHISMQ2

GENETICQ2

PERSIAN GULFQ2

OMAN SEAQ2

Abstract

Background and Aim: Given the nutritious value of COBIA and its importance in terms of rapid growth in cultural conditions and noticeable commercial value, along with lack of sufficient literature in this regard, this study was run to investigate genomic POLYMORPHISM of this species in southern waters of Iran in 2010. The study aimed at enlightening GENETIC variation and determining its rate inside and between probable populations.Materials and Methods: This cross sectional study was carried out on 120 specimens of COBIA which were randomly obtained from four southern Iranian provinces. Two-gram cuts of fin tissue of each fish were transferred to the laboratory in 2 ml microtubes containing ethanol. DNA was extracted by Phenol-chlorophorm method and genomic POLYMORPHISM investigation was carried out by PCR-RFLP method. In this method, PCR product of COI mitochondrial gene were enzymatic digested using 6 Restriction Enzymes.Results: PCR product of mitochondrial gene COI was obtained in length of 1060 base pair and 6 polymoiphic sites were investigated. But electrophoresis patterns of enzymatic digestion products were monomorphous in all samples and no POLYMORPHISM was observed.Conclusion: Lack of GENETIC diversity or its noticeable decrease in regions where fish can freely migrate and move between two regions has been reported. Due to lack of physical and biological obstacles Dobia is able to migrate to all parts of the PERSIAN GULF and OMAN SEA. Therefore, lack of POLYMORPHISM in the studied COBIA population seems to be logical. Future studies with larger samples and more enzymes from other GENETIC regions are recommended.

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APA: Copy

TALA, MARYAM, KAZEMI DAMANE, BAHRAM, LALOIE, FARAMARZ, SOLTANI, MEHDI, AZAD, MANSOOR, & KOOCHAKI, AMENEH. (2012). INVESTIGATION ON POLYMORPHISM OF MITOCHONDRIAL GENOME IN COBIA FISH RACHYCENTRON CANADUM IN THE NORTHERN WATERS OF THE PERSIAN GULF AND OMAN SEA. RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH), 16(5 (83)), 246-251. SID. https://sid.ir/paper/18329/en

Vancouver: Copy

TALA MARYAM, KAZEMI DAMANE BAHRAM, LALOIE FARAMARZ, SOLTANI MEHDI, AZAD MANSOOR, KOOCHAKI AMENEH. INVESTIGATION ON POLYMORPHISM OF MITOCHONDRIAL GENOME IN COBIA FISH RACHYCENTRON CANADUM IN THE NORTHERN WATERS OF THE PERSIAN GULF AND OMAN SEA. RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH)[Internet]. 2012;16(5 (83)):246-251. Available from: https://sid.ir/paper/18329/en

IEEE: Copy

MARYAM TALA, BAHRAM KAZEMI DAMANE, FARAMARZ LALOIE, MEHDI SOLTANI, MANSOOR AZAD, and AMENEH KOOCHAKI, “INVESTIGATION ON POLYMORPHISM OF MITOCHONDRIAL GENOME IN COBIA FISH RACHYCENTRON CANADUM IN THE NORTHERN WATERS OF THE PERSIAN GULF AND OMAN SEA,” RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH), vol. 16, no. 5 (83), pp. 246–251, 2012, [Online]. Available: https://sid.ir/paper/18329/en

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