EFFECTS OF PRECOOLING AND GROWTH REGULATORS ON MICROPROPAGATION OF ESTAHBAN SAFFRON (CROCUS SATIVUS L.)

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SHARAFZADEH SH.; KHOUSHKHOUI MORTEZA

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Journal: IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY Year:2004 | Volume:5 | Issue:3 Page(s): 129-136

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Title

EFFECTS OF PRECOOLING AND GROWTH REGULATORS ON MICROPROPAGATION OF ESTAHBAN SAFFRON (CROCUS SATIVUS L.)

Author(s)

SHARAFZADEH SH. | KHOUSHKHOUI MORTEZA | Issue Writer Certificate

Keywords

IN VITRO PROPAGATIONQ4

MICROPROPAGATIONQ4

SAFFRONQ3

TISSUE CULTUREQ2

Abstract

Saffron is one of the native plants of Iran. In this investigation, suitable culture medium and growth regulator requirements for IN VITRO PROPAGATION of SAFFRON were studied. Corms of ‘Estahban’ SAFFRON were planted for TISSUE CULTURE studies at College of Agriculture, Shiraz University. Half of corms were kept at 4-5°C and the others at 15-30 oC. Half-strength Murashige and Skoog (MS) medium supplemented with 0, 0.5, 1 and 1.5 mg l-1 2, 4-D and BA, 500 mg l-1 casein hydrolysate and 50 g 1-1 sucrose was used for cormlet production. In vitro grown cormlets were transferred to MS medium with 1.4, 1.6, 1.8, 2.0, 2.2, 2.4, 2.6, 2.8 and 3.0 mg l-1 NAA for further growth. A half-strength MS medium supplemented with 0, 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 mg l-1 BA and NAA was used for shoot production experiments. Number of in vitro produced cormlets, cormlet diameter and shoot length were recorded after 3, 6 and 8 weeks, respectively. Results indicated that low temperature pretreatment is necessary for in vitro cormlet production and larger corms produced more cormlets. 0.5 mg l-1 BA and 1 mg l-1 2, 4-D were optimum concentrations for cormlet production. Similarly, 1.8 mg l-1 NAA for cormlet growth and 10 mg l-1 NAA and 10 mg l-1 BA for shoot production were the optimum concentrations.

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APA: Copy

SHARAFZADEH, SH., & KHOUSHKHOUI, MORTEZA. (2004). EFFECTS OF PRECOOLING AND GROWTH REGULATORS ON MICROPROPAGATION OF ESTAHBAN SAFFRON (CROCUS SATIVUS L.). IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, 5(3), 129-136. SID. https://sid.ir/paper/80771/en

Vancouver: Copy

SHARAFZADEH SH., KHOUSHKHOUI MORTEZA. EFFECTS OF PRECOOLING AND GROWTH REGULATORS ON MICROPROPAGATION OF ESTAHBAN SAFFRON (CROCUS SATIVUS L.). IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY[Internet]. 2004;5(3):129-136. Available from: https://sid.ir/paper/80771/en

IEEE: Copy

SH. SHARAFZADEH, and MORTEZA KHOUSHKHOUI, “EFFECTS OF PRECOOLING AND GROWTH REGULATORS ON MICROPROPAGATION OF ESTAHBAN SAFFRON (CROCUS SATIVUS L.),” IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, vol. 5, no. 3, pp. 129–136, 2004, [Online]. Available: https://sid.ir/paper/80771/en

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