CELLULAR EFFECTS OF ANTIBACTERIAL DRUGS, SULFATHIAZOLE AND SULFACETAMIDE, ON A BREAST CANCER CELL LINE (T-47D)

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MOHAMMADPOUR R.; SAFARIAN S.; NOROUZI S.; RAZAZAN A.

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Journal: Journal of Molecular and Cellular Researches Year:2014 | Volume:26 | Issue:4 Page(s): 532-549

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Title

CELLULAR EFFECTS OF ANTIBACTERIAL DRUGS, SULFATHIAZOLE AND SULFACETAMIDE, ON A BREAST CANCER CELL LINE (T-47D)

Author(s)

MOHAMMADPOUR R. | SAFARIAN S. | NOROUZI S. | RAZAZAN A. | Issue Writer Certificate

Keywords

SULFATHIAZOLEQ2

SULFACETAMIDEQ2

BREAST CANCERQ1

APOPTOSISQ1

CELL CYCLE ARRESTQ2

AUTOPHAGYQ1

Abstract

Primary anti-bacterial drugs, called sulfonamide drugs, have several biological effects drived widespread use of these drugs in medicine. Here, with respect to some reports (confirming anti-cancerous activity of some sulfadrugs) cellular effects of two antibacterial sulfonamides (SULFATHIAZOLE and SULFACETAMIDE) on T-47D cells was studied using several experimental methods. The fifty percent reduction in cell viability (LC50) was investigated after 48 h treatment of T-47D cells with sodium SULFATHIAZOLE and sodium SULFACETAMIDE. Cells undergoing APOPTOSIS were identified using fluorescent microscopy after double staining with Annexin-PI fluorochromes. Flow cytometric analysis, using Annexin-V-Flous kit, revealed lack of APOPTOSIS amoung the treated cells. This notion was also exhibited by DNA laddering investigations while increasing in caspases-3 activity was registered. Cell cycle analysis after DAPI treatment indicated lack of CELL CYCLE ARREST amoung the treated cells. Here, doxorubicin was utilized as a positive control in all experiments. On the whole, regarding to the present study, it has been exhibited that in the presence of sodium SULFATHIAZOLE, decreased transcriptional expression level of Akt1, Akt2 and mTOR genes, in parallel with increased transcriptional level of PTEN gene, is being followed by reducing of cell viability. No similar mechanism was observed for sodium SULFACETAMIDE. With respect to increased transcriptional expression level of Atg5, Akt1, Akt2 and mTOR genes, in parallel with decreased transcription level of PTEN gene, mechanism of action of sodium SULFACETAMIDE should be exerted by cytoprotective AUTOPHAGY causing decreasing of cell devision or growth rate.

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APA: Copy

MOHAMMADPOUR, R., SAFARIAN, S., NOROUZI, S., & RAZAZAN, A.. (2014). CELLULAR EFFECTS OF ANTIBACTERIAL DRUGS, SULFATHIAZOLE AND SULFACETAMIDE, ON A BREAST CANCER CELL LINE (T-47D). JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 26(4), 532-549. SID. https://sid.ir/paper/248407/en

Vancouver: Copy

MOHAMMADPOUR R., SAFARIAN S., NOROUZI S., RAZAZAN A.. CELLULAR EFFECTS OF ANTIBACTERIAL DRUGS, SULFATHIAZOLE AND SULFACETAMIDE, ON A BREAST CANCER CELL LINE (T-47D). JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2014;26(4):532-549. Available from: https://sid.ir/paper/248407/en

IEEE: Copy

R. MOHAMMADPOUR, S. SAFARIAN, S. NOROUZI, and A. RAZAZAN, “CELLULAR EFFECTS OF ANTIBACTERIAL DRUGS, SULFATHIAZOLE AND SULFACETAMIDE, ON A BREAST CANCER CELL LINE (T-47D),” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 26, no. 4, pp. 532–549, 2014, [Online]. Available: https://sid.ir/paper/248407/en

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