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Information Journal Paper

Title

The effects of 2, 4-D pretreatment and plant growth regulators on tomato (Solanum lycopersicum L. ) regeneration

Pages

  35-48

Abstract

 Because of the importance of tomato in the human diet, a reproducible in vitro regeneration system with commercial applications is necessary for its genetic transformation. Active cellular division at the time of inoculation of plant tissues by Agrobacterium, has a crucial role in plant transformation efficiency. To achieving this purpose, the effects of an auxin pretreatment, flowed with other plant growth regulator treatments on tomato organogenesis was investigated. Cotyledon and hypocotyl explants were prepared from seven day seedlings and were placed on MS medium supplemented with 3% sucrose and 0. 6% agar and different concentrations of IAA, NAA and BAP. Pretreatment of explants with 2, 4-D had no effect on plant regeneration; but lead to callus formation. Calli didn’ t produce shoot in other mediums. The highest percentage of regeneration was observed in Cherry cultivar in mediums containing BAP (3 or 1. 5 mg/l) + 0. 1 mg/l IAA. The highest shoot formation was noted in cotyledon explants of Cherry tomato in MS mediums supplemented with 3 or 1. 5 mg/l BAP + 0. 1 mg/l IAA. Rooting of shoots was done in medium containing 2 mg/l IBA.

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    APA: Copy

    Aziz Khajeh, a., & Dorani, e.. (2018). The effects of 2, 4-D pretreatment and plant growth regulators on tomato (Solanum lycopersicum L. ) regeneration. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 31(1 ), 35-48. SID. https://sid.ir/paper/248418/en

    Vancouver: Copy

    Aziz Khajeh a., Dorani e.. The effects of 2, 4-D pretreatment and plant growth regulators on tomato (Solanum lycopersicum L. ) regeneration. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2018;31(1 ):35-48. Available from: https://sid.ir/paper/248418/en

    IEEE: Copy

    a. Aziz Khajeh, and e. Dorani, “The effects of 2, 4-D pretreatment and plant growth regulators on tomato (Solanum lycopersicum L. ) regeneration,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 31, no. 1 , pp. 35–48, 2018, [Online]. Available: https://sid.ir/paper/248418/en

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