AN INVESTIGATION OF THE EFFECT OF COPPER NANOPARTICLES ON STAPHYLOCOCCUS AUREUS GENOME BY RAPD MOLECULAR MARKERS

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ZARE N.; GOLESTANI EMANI B.; KARIMI F.

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Paper Information

Journal: Journal of Molecular and Cellular Researches Year:2017 | Volume:30 | Issue:1 Page(s): 14-25

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Information Journal Paper

Title

AN INVESTIGATION OF THE EFFECT OF COPPER NANOPARTICLES ON STAPHYLOCOCCUS AUREUS GENOME BY RAPD MOLECULAR MARKERS

Author(s)

ZARE N. | GOLESTANI EMANI B. | KARIMI F. | Issue Writer Certificate

Keywords

NANOPARTICLES COPPER OXIDEQ1

STAPHYLOCOCCUS AUREUSQ1

RAPID POLYMERASE CHAIN REACTION(RAPD-PCR)Q1

Abstract

Regarding the bacterial Resistance of common Antibiotics and anti-microbial agents, a great number of studies have been conducted to discover the recent types of anti-microbial agents. With the recent developments in nanotechnology, copper has been extensively used as nanoparticles against all positive-gram and to negative-gram particles due to its low price and high anti-microbial activity. In the present study, copper oxide nanoparticles of (<20 nm) have been used to study its effect on the genome of STAPHYLOCOCCUS AUREUS as a model for Gram-positive bacteria. To this end, the bacteria were first treated with 30 and 60 mg/mL copper oxide nanoparticles at the intervals of 2, 4, and 24 hours. And then their DNA were extracted. In order to investigate the effects of copper oxide nanoparticles on the genome, the chain reaction techniques of (RAPD-PCR) was employed. Using the software NTSYS-PC, the results obtained from electrophoresis of PCR products on Agarose gel were analyzed. The results of the study revealed that copper oxide nanoparticles not only effect the growth of Bacteria but also affect the sequencing of genomic DNA and leads to the changes of them in different points.

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