Journal of Molecular and Cellular Researches
Year:2017 | Volume:30 | Issue:1 |Papers Count:10

Dracocephalum kotschyi Boiss belonging to Labiateae family is an herbaceous, pereninal and endemic plant known in Iran as Zarrin-Giah. Recent pharmacological studies demonstrated this plants with methoxylated flavonoids, have anti-cancer effects.Hairy roots were obtained from leaves explants, inoculated with 15834 strain of Agrobacterium rhizogenesis. In present study, The effects of biotic elicitors including, Colchicine with different cocentrations (0, 0.01, 0.03 and 0.05% (w/v)) and Chitosan (0, 50, 100 and 150 mg/l) for 48 hours on hairy root growth and Rosmarinic acid production were investigated. ANOVA results of Antioxidant activity, total phenol and total flavonoids showed significant difference among the various treatments (P<0.01).maximum Antioxidant activity (90.23%) was observed in 0.05% colchicine and lowest Antioxidant activity (63%) was observed in non-transformed roots. HPLC analysis revealed that the maximum Rosmarinic acid content (35.8 and 15.1 mg /mg DW) obtained in colchicine (0.05%) and chitosan (100 mg/l) respectively. Although minimum Rosmarinic acid content (13.8 mg /mg DW) were recorded in no transformed roots. based on the importance of rosmarinic acid production in the pharmaceutical industry, the results showed that using biotechnological methods, production improving of valuable medicinal substances is acceptable.

Regarding the bacterial Resistance of common Antibiotics and anti-microbial agents, a great number of studies have been conducted to discover the recent types of anti-microbial agents. With the recent developments in nanotechnology, copper has been extensively used as nanoparticles against all positive-gram and to negative-gram particles due to its low price and high anti-microbial activity. In the present study, copper oxide nanoparticles of (<20 nm) have been used to study its effect on the genome of Staphylococcus aureus as a model for Gram-positive bacteria. To this end, the bacteria were first treated with 30 and 60 mg/mL copper oxide nanoparticles at the intervals of 2, 4, and 24 hours. And then their DNA were extracted. In order to investigate the effects of copper oxide nanoparticles on the genome, the chain reaction techniques of (RAPD-PCR) was employed. Using the software NTSYS-PC, the results obtained from electrophoresis of PCR products on Agarose gel were analyzed. The results of the study revealed that copper oxide nanoparticles not only effect the growth of Bacteria but also affect the sequencing of genomic DNA and leads to the changes of them in different points.

Wounds are one of the clinical problems and can be seen in the form of acute or chronic, due to its importance wound care management and medical treatment is a priority. All the products and processes that can minimize healing time can help to solve the problems of patients. Now a day new look to traditional medicine and biological products as opposed to synthetic drugs has emerged in this case. In this study the effect of Lucilia sericata maggot extract on wound healing were studied, for the purpose the extract was evaluated by MTT assay to assess itsin vitro effect on cell growth and its effect on cell migration andin vivo effects in created mouse skin wound healing were also evaluated. The study shown that IC50 was not detected for 24 hours but for 48 and 72 hours were 28.7 and 23.45 microgram per milliliter respectively. Results showed that best healing can take place during first 24 hours using 12.5 microgram per milliliter, resulting in the induction of cell proliferation and cell migration but during 48 hours this effect get decreased and in 72 hours resulted to cell growth inhibition. And the results of animal modeling also showed that the 12.5 microgram per milliliter extract promotes mice wound healing. According to the results of this study and the positive effect of the extract within 24 hours on skin wound healing and also cell growth and because of some physical as well as psychotherapists hardness in live larva administration on the wounds of patients, it is suggested study get expand and experimental level then be studied on human wounds.

One ways to increase the operational efficiency of leaching metals from sulfide minerals is using efficient species of sulfur oxidizing bacteria in metal mining. Thus, the aim of this study was to isolate and identify sulfur oxidizing bacteria from soils of Sarcheshmeh copper mine (Kerman, south of Iran) and to survey their operation in sulfur oxidation. After enrichment, samples were purified and then isolates were identified based on their morphological characteristics and phylogenic techniques.Subsequently, three sulfur-oxidizing strains which belonged to Acidithiobacillus sp and Sulfobacillus spwere isolated and identified. Of these three, strain 184, with 95% similarity toAcidithiobacillus ferridurans was selected as the superior strain due to higher growth rate (change of 2 value pH and 385 mg S/L during 2 weeks) in the synthetic mineral medium containing sulfur which can now be used as an applicable strain in bioleaching of copper metal.

Nanomaterials are a part from an industrial revolution to develop lightweight but strong materials for a variety of purposes. The toxic effects of nanoparticles have been evaluated in a variety of studies; however the potential health and environmental impacts on plants have yet to be thoroughly examined. This research were performed to evaluation the nano silver effect on cytogenetical indices of deviation cells in 6 barely cultivars. Seeds germination in various concentrations of nano silver (0, 1 percent, 5 percent and 10 percent Molar) was performed in factorial design based on completely randomized design. The traits of mitotic index, metaphase index and chromosome deviation index were measured. The results of variance analysis were showed a significant difference between various concentrations of nano particle for studied characters at 1 percent level. Significant difference was observed between cultivars only for mitotic index at 5 percent level. The compare means by Duncan's methods revealed that the traits of mitotic index and metaphase index were significantly increased by increasing concentrations of nanoparticles up to 5 percent and at 10 percent concentration, a significant decrease were observed for both indices. For the chromosome deviation index the highest level were observed for 1 percent concentration and the chromosome deviation index were significantly decreased at the 5 percent and 10 percent concentrations. In addition, in barley cultivars, the lowest concentrations of nanoparticles (1 percent and 5 percent) increased the mitotic index, metaphase index and chromosome deviation index and 10 percent concentration were decrease the amount of studied indices, compared to check treatment. It seems that nano silver has potential of creating damage to genetic material and attention to security aspects and environmental has specific importance.

Some bacteria present a capability in bioremediation of oil derivatives. In this study the bacteria existing in the plant rhizospere of polluted soils were isolated and purified.These bacteria were then exposed to a combination of commonly used petroleum products with different concentration. The bacteria that capable of biodegrading these compounds, were selected and determined using taxonomical keys and biochemical tests. Their efficiencies in removing the petroleum derivatives were compared. Finally, bacteria with the highest capability for degradation of all oil products were introduced.According to our results, Alcaligenese utrupha, Pseudomonas alcaligenes and Pseudomonas aeroginosawere isolated from rhizosphere of the soils contaminated by kerosene and gasoil; Pseudomonas alcaligenes, Bacillus coagulans, and Bacillus circulanswere isolated from soils contaminated by kerosene and engine oil; and Pseudomonas alcaligeneswas isolated from soils contaminated by gasoil and engine oil.Pseudomonas alcaligenes was able to biodegrade the all types of the studied oil products. The bioremediation efficiencies of this bactrium were 72, 63 and 56% repectively.

Investigate the effects of salt stress on some physiological and morphological traits in four varieties and four salinity levels, including SC302, SC700, BC662, SC704 and Zero (control), 50, 100 and 150 mM NaCl in three replicates for the factorial experiment in randomized complete block design was carried out. During the experiment, several traits including plant height, Stem diameter, chlorophyll a, chlorophyll b, proline and antioxidant enzymes were measured. Between the different salinity in the chlorophyll a was seen significant difference. In The chlorophyll b, among different salinities, genotypes and interactions between them no significant difference was found. Between different levels of salinity, a significant difference was seen in catalase. Between genotypes of the enzyme ascorbate peroxidase significant difference was seen. Highest proline was determined in 100 mM NaCl, in SC302 with 537 micro mol. The most plant height was obtained at zero salinity in S.C704 and B.C662. There was no significant difference between them. Maximum chlorophyll a in normal conditions was found in S.C704 with 1.27 mg of chlorophyll per gram fresh weight of leaves. The highest superoxide dismutase, were obtained in 50 mM NaCl in SC700. With increasing salinity, the amount of catalase increased and at 100 mM salt stress reached a maximum. Maximum catalase was measured in 100 mM salt in BC662, which with SC302 and SC700 was not significantly different. Of the enzyme ascorbate peroxidase, the enzyme in the greatest amount of salt concentration in 50 mmol in Sc302 with 3.73 was obtained.

Tang Fany petroleum Pumping Station (TFOPS) of Poldokhtar is an important and the old point of oil transition. The soil and water around this site is petroleum polluted due leakage of petroleum. The purpose of this study was to evaluate effective factors for survival and fitness of the petroleum degrading bacteria. The following bacterial species were isolated and identified from petroleum polluted soil and water in TFOPS region: Pseudomoas aeruginosa, Acinetobacter junii, Acinetobacter buumanii, Delftia tsuruhatensis, Comamonas Koreensis, Sphinobacterium multivorum and Stenotrophomonas acidaminiphila. The MIC and MBC were determined relative to oil whit the macrodilution method. The bacterial species Compared and evaluated on the factors ability to form a bacterial biofilm, siderophore production, indole acetic acid and dissolve phosphate. Two species namelyP. aeruginosa and C. koreensis showed the highest and the least petroleum concentration tolerance, respectively. The two species also have the highest and the least biofilm production ability, respectively.Acinetobacter juniiand A. baumannii in the test of dissolution of phosphate, most were able to dissolve phosphate.Sphingobacterium multivorum highest produced indole acetic acid. Due to the ability of the bacterial species to tolerate oil pollution and factors to promote plant growth, it can be suggested as alternative for application in the Bioremediation of contaminated soil events.

Thermolysin, obtained frombacillus thermoproteolyticus, is a thermophilic protease having application in peptide synthesis. Previous studies have revealed that salt activates the enzyme. Thermal stability of TLN is critically dependent on four calcium ions bound in N- and C-terminal domains. The aim of present study is unraveling the role of calcium ions in the salt activation of enzyme. In the presence of NaCl2 M, measurement of the activity of enzyme in the absence and presence of CaCl2 10 mM using FAGLA as substrate was investigated. In addition, Far-UV circular dichroism and fluorescence spectroscopies were used to understand the calcium dependent changes in the enzyme structure. Results revealed the activation of enzyme using FAGLA and in the presence of CaCl2 10 mM increased 2.5 times. Far-UV circular dichroism spectra suggest that the overall structure of thermolysin has not changed during salt and calcium activation. However, fluorescence studies revealed that the active site has changed under calcium-dependent salt activation. According to the results of the present study, the binding of calcium ions might influence the activation of thermolysin by salt.

In this study, Population of Garra rufa was investigated using six microsatellite loci in Fars and Kohgiluyeh and Boyer-Ahmad province (Shahpour and Berim Rivers). In this study fulfilled on 60 Garra individuals (30 individuals for each river). The mean number of allele’s level at the population was 13.5 which are more than the reported values for freshwater fishes. The expected and observed heterozygosity were 0.658 and 0.865, respectively. Most cases deviated significantly from Hardy-Weinberg equilibrium (p£0.01). It seems that there is a low differentiation between two populations of this species in the studied areas due to high level of gene flow (Nm=18.205) between two regions and low Fst (0.036). Also, analysis of molecular variance showed there is low genetic differentiation among populations and most of the observed variation is within the populations.