COMPARATIVE STUDIES ON THE ORGANIC SOLVENT STABILITY OF ELASTASE FROM PSEUDOMONAS AERUGINOSA AND THERMOLYSIN FROM BACILLUS THERMOPROTEOLYTICUS

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SADREMOMTAZ A.; ASGHARI S.M.

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Journal: Journal of Molecular and Cellular Researches Year:2015 | Volume:28 | Issue:4 Page(s): 560-567

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Title

COMPARATIVE STUDIES ON THE ORGANIC SOLVENT STABILITY OF ELASTASE FROM PSEUDOMONAS AERUGINOSA AND THERMOLYSIN FROM BACILLUS THERMOPROTEOLYTICUS

Author(s)

SADREMOMTAZ A. | ASGHARI S.M. | Issue Writer Certificate

Keywords

METALOPROTEASEQ2

THERMOLYSINQ2

ELASTASEQ2

ORGANIC SOLVENTQ2

ACTIVITY OF ENZYMESQ2

Abstract

Extensive research has been conducted on the stability of proteases in ORGANIC SOLVENTs, because they have numerous applications in organic media. THERMOLYSIN fromBacillus thermoproteolyticusis a highly thermostable Zn-metalloprotease and is applied in industry for peptide synthesis, a reaction essentially performs in organic media. However, THERMOLYSIN has low stability at ORGANIC SOLVENTs.In contrast, its homologous enzyme, Pseudomonas aeruginosa ELASTASE (PAE) is an ORGANIC SOLVENT stable protease. In the present study, recombinant PAE was purified and compared with THERMOLYSIN in different ORGANIC SOLVENTs. For this purpose, ACTIVITY OF ENZYMES was measured in ethanol, methanol, isopropanol, dimethylformamide, glycerol and ethylenglycol (0-50 % (V/V)). Except isopropanol and ethanol, not only the ELASTASE activity was not decreased, but also strongly increased in ORGANIC SOLVENTs. However, activity of THERMOLYSIN was abolished in all ORGANIC SOLVENTs. In the presence of DTT 1mM, the PAE activity was notably decreased in ORGANIC SOLVENTs. Considering that PAE contains two disulfide bonds at N- and C-terminal domains, it may suggest that disulfide bonds play a role in ORGANIC SOLVENT stability of PAE.

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APA: Copy

SADREMOMTAZ, A., & ASGHARI, S.M.. (2015). COMPARATIVE STUDIES ON THE ORGANIC SOLVENT STABILITY OF ELASTASE FROM PSEUDOMONAS AERUGINOSA AND THERMOLYSIN FROM BACILLUS THERMOPROTEOLYTICUS. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 28(4), 560-567. SID. https://sid.ir/paper/248575/en

Vancouver: Copy

SADREMOMTAZ A., ASGHARI S.M.. COMPARATIVE STUDIES ON THE ORGANIC SOLVENT STABILITY OF ELASTASE FROM PSEUDOMONAS AERUGINOSA AND THERMOLYSIN FROM BACILLUS THERMOPROTEOLYTICUS. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2015;28(4):560-567. Available from: https://sid.ir/paper/248575/en

IEEE: Copy

A. SADREMOMTAZ, and S.M. ASGHARI, “COMPARATIVE STUDIES ON THE ORGANIC SOLVENT STABILITY OF ELASTASE FROM PSEUDOMONAS AERUGINOSA AND THERMOLYSIN FROM BACILLUS THERMOPROTEOLYTICUS,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 28, no. 4, pp. 560–567, 2015, [Online]. Available: https://sid.ir/paper/248575/en

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