RECOMBINANT CHITINASE PRODUCED FROM A THERMOPHILIC PAENIBACILLUS SP. A01

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MOTAZAVI M.; AMINZADEH S.; GHANBARI A.R.; FARROKHI N.; KARKHANE A.A.; JAVAHERI SAFA Z.

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Journal: Journal of Molecular and Cellular Researches Year:2017 | Volume:30 | Issue:2 Page(s): 182-190

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Title

RECOMBINANT CHITINASE PRODUCED FROM A THERMOPHILIC PAENIBACILLUS SP. A01

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Keywords

CHITINQ1

CHITINASEQ2

THERMOPHILIC BACTERIUMQ2

PAENIBACILLUS EHIMENSISQ1

Abstract

CHITIN is the second most abundant organic polymer in nature after cellulose and it is the main part of Insects cuticle and crustaceans and includes in cell wall of most fungus and some algae and nematodes. Huge amounts of CHITIN residual are being produced by organisms that enter to the nature as natural pollutants. However, this polysaccharide has immense application in different aspects of our daily life once becomes digested via CHITINases. CHITINase is one of the enzymes that responsible for disintegrating CHITIN. Some of bacteria produce CHITINase for digesting CHITIN. Here, a thermophilic CHITINase gene (JQ675723) obtained from a PAENIBACILLUS EHIMENSIS isolate. The gene was cloned in pET26b and transformed into E. coli to heterologously produce the enzyme. The recombinant protein was isolated via affinity chromatography using Ni-NTA column. The enzyme demonstrated to have hydrolytic activity in the presence of CHITIN and by addition of 3, 5-dinitrosalicylic acid at 530 nm. Moreover high temperature showed industrial potential. Its nucleotide sequence had high similarity to Glycoside hydrolase family 18. Amino acid sequence from amino to carboxyl determined glycosyl hydrolase domain, fibronectin like domain and CHITIN binding domain.

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APA: Copy

MOTAZAVI, M., AMINZADEH, S., GHANBARI, A.R., FARROKHI, N., KARKHANE, A.A., & JAVAHERI SAFA, Z.. (2017). RECOMBINANT CHITINASE PRODUCED FROM A THERMOPHILIC PAENIBACILLUS SP. A01. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 30(2 ), 182-190. SID. https://sid.ir/paper/248593/en

Vancouver: Copy

MOTAZAVI M., AMINZADEH S., GHANBARI A.R., FARROKHI N., KARKHANE A.A., JAVAHERI SAFA Z.. RECOMBINANT CHITINASE PRODUCED FROM A THERMOPHILIC PAENIBACILLUS SP. A01. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2017;30(2 ):182-190. Available from: https://sid.ir/paper/248593/en

IEEE: Copy

M. MOTAZAVI, S. AMINZADEH, A.R. GHANBARI, N. FARROKHI, A.A. KARKHANE, and Z. JAVAHERI SAFA, “RECOMBINANT CHITINASE PRODUCED FROM A THERMOPHILIC PAENIBACILLUS SP. A01,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 30, no. 2 , pp. 182–190, 2017, [Online]. Available: https://sid.ir/paper/248593/en

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