Isolation and Identification of an Alkaliplilic Thermo-tolerant Protease producing Bacillus sp. from Dehloran Hot Spring: Production Optimization and Investigation of the Activity and Stability of the Enzyme

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MEHRABI MARYAM; NAZARI LALEH; ROSTAMI ZHILA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Microbial Biology Year:2019 | Volume:8 | Issue:29 Page(s): 83-96

Download Full-Text

Persian Verion

View:

569

Download:

Cites:

Information Journal Paper

Title

Isolation and Identification of an Alkaliplilic Thermo-tolerant Protease producing Bacillus sp. from Dehloran Hot Spring: Production Optimization and Investigation of the Activity and Stability of the Enzyme

Author(s)

MEHRABI MARYAM | NAZARI LALEH | ROSTAMI ZHILA | Issue Writer Certificate

Keywords

ProteaseQ2

Hot springQ1

OptimizationQ2

BacillusQ2

Abstract

Introduction: Thermophilic Proteases can be used in various industries, including detergents, pharmaceuticals, food, etc. These enzymes are produced by thermophilic microorganisms, including bacteria. Materials and methods: Sampling was carried out from Dehloran Hot springs in Ilam province in the west of Iran to find new Protease producing bacteria. Then, the effect of pH, temperature and finally the effect of different heating time on the production of Protease enzyme were evaluated. Then, Polymerase Chain Reaction (PCR) was performed to detect the strain. Protease was purified through anion exchange using DEAE-sepharose column and its molecular weight was estimated using SDS-PAGE technique. Then, activity and stability of the enzyme were investigated in temperature and pH range. Results: Among isolated strains, bacteria Bacillus sp. DEM07 (registered in the World Gene Bank with access number KY392988) with the highest diameter of the Protease clear zone, was selected to produce thermo tolerant alkaline Protease. The maximum production of the alkaline Protease enzyme was observed at 50 ° C, pH 7 and 48 hours after culture. The Protease enzyme was purified by anionic chromatography and its molecular weight was estimated to be about 27. 5 kDa after purification. The enzyme was active and stable at the temperature range of 30 to 55 ° C and the optimum temperature of the enzyme activity was observed at 50 ° C. The pH range for activity and its stability was from 4 to 11, and the optimum activity of the enzyme was observed at pH 10. Discussion and conclusion: In this study, the Protease enzyme purified from Bacillus sp. DEM07 is a thermo tolerant alkalophilic Protease. On the other hand, by creating the optimal conditions for achieving high production of thermo-tolerant alkalophilic Protease, this enzyme has a high potential for use in various industries.

Cites

No record.

References

No record.

Cite

APA: Copy

MEHRABI, MARYAM, NAZARI, LALEH, & ROSTAMI, ZHILA. (2019). Isolation and Identification of an Alkaliplilic Thermo-tolerant Protease producing Bacillus sp. from Dehloran Hot Spring: Production Optimization and Investigation of the Activity and Stability of the Enzyme. BIOLOGICAL JOURNAL OF MICROORGANISM, 8(29 ), 83-96. SID. https://sid.ir/paper/237339/en

Vancouver: Copy

MEHRABI MARYAM, NAZARI LALEH, ROSTAMI ZHILA. Isolation and Identification of an Alkaliplilic Thermo-tolerant Protease producing Bacillus sp. from Dehloran Hot Spring: Production Optimization and Investigation of the Activity and Stability of the Enzyme. BIOLOGICAL JOURNAL OF MICROORGANISM[Internet]. 2019;8(29 ):83-96. Available from: https://sid.ir/paper/237339/en

IEEE: Copy

MARYAM MEHRABI, LALEH NAZARI, and ZHILA ROSTAMI, “Isolation and Identification of an Alkaliplilic Thermo-tolerant Protease producing Bacillus sp. from Dehloran Hot Spring: Production Optimization and Investigation of the Activity and Stability of the Enzyme,” BIOLOGICAL JOURNAL OF MICROORGANISM, vol. 8, no. 29 , pp. 83–96, 2019, [Online]. Available: https://sid.ir/paper/237339/en

Related Journal Papers