CYTOTOXICITY EVALUATION OF PRO ROOT MTA, ROOT MTA AND PORTLAND CEMENT (PC) ON L929 MOUSE FIBROBLASTS

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SHARIFIAN MOHAMMAD REZA; ZARABIAN M.; RAZMI HASAN; GHOBADI MEHR NOUSH; KHARAZIFARD M.J.; HEMATZADEH F.; AFZALIFAR R.

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Journal: Journal of Dental Medicine Year:2007 | Volume:20 | Issue:2 (51) Page(s): 131-137

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Title

CYTOTOXICITY EVALUATION OF PRO ROOT MTA, ROOT MTA AND PORTLAND CEMENT (PC) ON L929 MOUSE FIBROBLASTS

Author(s)

Keywords

CYTOTOXICITYQ2

MINERAL TRIOXIDE AGGREGATE (MTA)Q3

PRO ROOT MTAQ3

ROOT MTAQ3

PORTLAND CEMENT (PC)Q3

L929 FIBROBLASTSQ3

Abstract

Background and Aim: Mineral Trioxide Aggregate (MTA) is a material used in many endodontic problems.Recently a number of studies have reported that Portland Cement (PC) and MTA have similar physical, chemical and biologic properties. In addition, a material known as ROOT MTA which is produced in Iran has been claimed to have similar properties to ROOT MTA/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank"> PRO ROOT MTA. If these claims are true, possible use of PC and ROOT MTA in clinic instead of ROOT MTA/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank"> PRO ROOT MTA will be quite cost effective. The aim of this study was to investigate the toxicity of ROOT MTA/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank"> PRO ROOT MTA, ROOT MTA and Portland Cement on L929 mouse fibroblasts.Materials and Methods: In this experimental study 0, 4, 24 hours and 7 days' extracts of materials were transferred to cell culture plates containing L929 FIBROBLASTS. After 24 hours incubation, cells were stained by Neutral Red (NR), and optical density (OD) of each cell was read with ELISA reader. Data were analyzed using Tukey HSD and one way analysis of variance. P<0.05 was considered as the level of significance.Results: In all surveyed groups and negative control group, at all time points separated cells from the base of the well were round. Refraction which is a characteristic of cellular death was not observed, whereas the separated cells from the base of well in positive control group showed refractional characteristic.Conclusion: Based on the findings of this study ROOT MTA/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank"> PRO ROOT MTA, PC and ROOT MTA have the same biocompatibility. PC seems to have the potential to be used in the same clinical situation as MTA. However in order to replace MTA with these less expensive materials more in vitro and in vivo studies are suggested.

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APA: Copy

SHARIFIAN, MOHAMMAD REZA, ZARABIAN, M., RAZMI, HASAN, GHOBADI, MEHR NOUSH, KHARAZIFARD, M.J., HEMATZADEH, F., & AFZALIFAR, R.. (2007). CYTOTOXICITY EVALUATION OF PRO ROOT MTA, ROOT MTA AND PORTLAND CEMENT (PC) ON L929 MOUSE FIBROBLASTS. JOURNAL OF DENTAL MEDICINE, 20(2 (51)), 131-137. SID. https://sid.ir/paper/25156/en

Vancouver: Copy

SHARIFIAN MOHAMMAD REZA, ZARABIAN M., RAZMI HASAN, GHOBADI MEHR NOUSH, KHARAZIFARD M.J., HEMATZADEH F., AFZALIFAR R.. CYTOTOXICITY EVALUATION OF PRO ROOT MTA, ROOT MTA AND PORTLAND CEMENT (PC) ON L929 MOUSE FIBROBLASTS. JOURNAL OF DENTAL MEDICINE[Internet]. 2007;20(2 (51)):131-137. Available from: https://sid.ir/paper/25156/en

IEEE: Copy

MOHAMMAD REZA SHARIFIAN, M. ZARABIAN, HASAN RAZMI, MEHR NOUSH GHOBADI, M.J. KHARAZIFARD, F. HEMATZADEH, and R. AFZALIFAR, “CYTOTOXICITY EVALUATION OF PRO ROOT MTA, ROOT MTA AND PORTLAND CEMENT (PC) ON L929 MOUSE FIBROBLASTS,” JOURNAL OF DENTAL MEDICINE, vol. 20, no. 2 (51), pp. 131–137, 2007, [Online]. Available: https://sid.ir/paper/25156/en

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