FLUOROMETRIC DETERMINATION OF NITROFURANTOIN IN PLASMA

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EMAMI J.; AMINI K.; TAVAKOLI N.; HUSHFAR GH.A; NABI H.

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Journal: JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS) Year:2003 | Volume:7 | Issue:Supplement 2 Page(s): 103-109

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Title

FLUOROMETRIC DETERMINATION OF NITROFURANTOIN IN PLASMA

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Keywords

NITROFURANTIONQ4

FLUOROMETRYQ4

Abstract

Introduction. Following oral administration of nitrofurantoin (NTF), a nitrofuran antibiotic, blood concentrations are low due to rapid elimination of drug from the body. A sensitive analytical method is, therefore, required to quantify NTF blood levels for pharmacokinetic and bioequivalence studies. The aim of this study was, therefore, to develop a sensitive fluorometric assay for plasma NTF quantification using o-aminothiophenol to form a fluorescenet substance.Methods and Materials. Generally, sodium nitrate, o-aminothiophenol, and hydrochloric acid were added to 2 mL of sample containing NTF. The test tubes were then vortexed, heated in water bath and irradiated under a UV lamp. the fluorescence intensity (Fl) was subsequently measured at excitation of 375 and emission of 422 nm. To optimize the assay, the impact of solvents, type and concentration of acids, concentrations of o-aminothiophenol and sodium mitrate, the volume of organic solvents used to dissolve drug or reagents, reaction temperature, and surfactants were examined on the Fl. In order to further increase the assay sensitivity, the drug was extracted from plasma with ethylacetate and then subjected to the oputimized procedure.Results. Fl was enhanced when water was used as NTF solvent. Fl was increased with a concentration dependent manner once hydrochloric acid was used as medium acidifier. O-aminothiophenol resulted in greater Fl with a concentration dependent fashion. Considerabl Fl was also observed once volume of organic solvent reduced or the reaction temperature increased. The standard curve, covering a 50-2000 ng/mL concentration range, in plasma was linear (r2=0.999) and relative errors were within ± 14% and the CV% ranged from 2-16. Conclusions. The fluorescence method presented requires 2 mL of plasma, which can be conveniently taken from human subjects. The assay is relatively fast, sensitive, reliable, specific and applicable. The assay is especially useful for pharmacokonetic and bioequivalance studies of NTF in human beings.

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APA: Copy

EMAMI, J., AMINI, K., TAVAKOLI, N., HUSHFAR, GH.A, & NABI, H.. (2003). FLUOROMETRIC DETERMINATION OF NITROFURANTOIN IN PLASMA. JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS), 7(Supplement 2), 103-109. SID. https://sid.ir/paper/26678/en

Vancouver: Copy

EMAMI J., AMINI K., TAVAKOLI N., HUSHFAR GH.A, NABI H.. FLUOROMETRIC DETERMINATION OF NITROFURANTOIN IN PLASMA. JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS)[Internet]. 2003;7(Supplement 2):103-109. Available from: https://sid.ir/paper/26678/en

IEEE: Copy

J. EMAMI, K. AMINI, N. TAVAKOLI, GH.A HUSHFAR, and H. NABI, “FLUOROMETRIC DETERMINATION OF NITROFURANTOIN IN PLASMA,” JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS), vol. 7, no. Supplement 2, pp. 103–109, 2003, [Online]. Available: https://sid.ir/paper/26678/en

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