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Title

USE OF RANDOM AMPLIFIED POLYMORPHIC DNA POLYMERASE CHAIN REACTION (RAPD-PCR) AND ITS2 PCR ASSAYS FOR DIFFERENTIATION OF POPULATIONS AND PUTATIVE SIBLING SPECIES OF ANOPHELES FLUVIATILIS (DIPTERA: CULICIDAE) IN IRAN

Pages

  133-137

Abstract

 ANOPHELES FLUVIATILIS complex is known to be a vector of malaria in IRAN. Since mosquitoes of this species cover a wide geographical range in IRAN, they might have evolved into different separated populations. Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) assay was used to differentiate geographic populations of this species. DNA was extracted from individual mosquitoes from 8 localities in 4 south and southeast provinces and amplified in PCR reactions using 18 single primers of arbitrary nucleotide sequence. Results of RAPD-PCR showed that Kazeroun populations could simply be differentiated from other populations using a diagnostic fragment amplified with primer UBC-306. But other populations could not be differentiated either visually or by means of statistical analysis. Moreover ITS2 fragments of some selected specimens were amplified using a pair of universal primer and sequenced as a key standard for detection of putative sibling species. Sequence analysis of the ITS2 fragments revealed a very high (100%) homology among the populations. These findings are crucial in epidemiological studies concerning relatedness of geographic populations and vector movement in the region. Results of RAPD-PCR and ITS2 analysis suggest that this taxon in IRAN comprises of only one species with a low genetic variation among geographic populations.

Cites

References

Cite

APA: Copy

NADAF DEZFOULI, S.R., OSHAGHI, M.A., VATANDOUST, H., JAVADIAN, E., TELMADAREI, Z., & ASMAR, M.. (2002). USE OF RANDOM AMPLIFIED POLYMORPHIC DNA POLYMERASE CHAIN REACTION (RAPD-PCR) AND ITS2 PCR ASSAYS FOR DIFFERENTIATION OF POPULATIONS AND PUTATIVE SIBLING SPECIES OF ANOPHELES FLUVIATILIS (DIPTERA: CULICIDAE) IN IRAN. IRANIAN JOURNAL OF PUBLIC HEALTH, 31(3-4), 133-137. SID. https://sid.ir/paper/272183/en

Vancouver: Copy

NADAF DEZFOULI S.R., OSHAGHI M.A., VATANDOUST H., JAVADIAN E., TELMADAREI Z., ASMAR M.. USE OF RANDOM AMPLIFIED POLYMORPHIC DNA POLYMERASE CHAIN REACTION (RAPD-PCR) AND ITS2 PCR ASSAYS FOR DIFFERENTIATION OF POPULATIONS AND PUTATIVE SIBLING SPECIES OF ANOPHELES FLUVIATILIS (DIPTERA: CULICIDAE) IN IRAN. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2002;31(3-4):133-137. Available from: https://sid.ir/paper/272183/en

IEEE: Copy

S.R. NADAF DEZFOULI, M.A. OSHAGHI, H. VATANDOUST, E. JAVADIAN, Z. TELMADAREI, and M. ASMAR, “USE OF RANDOM AMPLIFIED POLYMORPHIC DNA POLYMERASE CHAIN REACTION (RAPD-PCR) AND ITS2 PCR ASSAYS FOR DIFFERENTIATION OF POPULATIONS AND PUTATIVE SIBLING SPECIES OF ANOPHELES FLUVIATILIS (DIPTERA: CULICIDAE) IN IRAN,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 31, no. 3-4, pp. 133–137, 2002, [Online]. Available: https://sid.ir/paper/272183/en

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