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Information Journal Paper

Title

EVALUATION OF A NATIVE PREPARATION OF HCV CORE PROTEIN (2-122) FOR POTENTIAL APPLICATIONS IN IMMUNIZATION, DIAGNOSIS AND MAB PRODUCTION

Pages

  1-10

Abstract

 Infection with HEPATITIS C virus (HCV) is a worldwide problem. Among HCV proteins, core antigen (Ag), besides its importance for diagnostic application is a prime candidate for component of a vaccine. Herein, we report results of studies on production of the hydrophilic domain of core Ag (2-122) in native conformation by an arabinose induction system in E.coli and the primary characterization of this recombinant protein for applications in diagnosis, immunization and mAb production. Recombinant core (r-Core) was able to detect anti-core antibodies in HCV positive serum samples in a dilution rate of 1/3200. It was also capable to elicit a potent anti-HCV humoral immune response in BALB/c mice. Finally, we established two stable clones of hybridoma which shown to produce specific and sensitive mAbs against the core protein. HCV core was able to elicit a broad range of antibody specificities depending on the immunogen conformation. Therefore, it may be possible to get new mAbs with higher affinities towards native conformation of core Ag.

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    APA: Copy

    AGHASADEGHI, M.R., SADAT, S.M., AMINI, SAFIEH, BAHRAMALI, G., ROUHVAND, F., BUDKOWSKA, A., KHABIRI, A.R., & NADAF, S.R.. (2006). EVALUATION OF A NATIVE PREPARATION OF HCV CORE PROTEIN (2-122) FOR POTENTIAL APPLICATIONS IN IMMUNIZATION, DIAGNOSIS AND MAB PRODUCTION. IRANIAN JOURNAL OF PUBLIC HEALTH, 35(1), 1-10. SID. https://sid.ir/paper/272227/en

    Vancouver: Copy

    AGHASADEGHI M.R., SADAT S.M., AMINI SAFIEH, BAHRAMALI G., ROUHVAND F., BUDKOWSKA A., KHABIRI A.R., NADAF S.R.. EVALUATION OF A NATIVE PREPARATION OF HCV CORE PROTEIN (2-122) FOR POTENTIAL APPLICATIONS IN IMMUNIZATION, DIAGNOSIS AND MAB PRODUCTION. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2006;35(1):1-10. Available from: https://sid.ir/paper/272227/en

    IEEE: Copy

    M.R. AGHASADEGHI, S.M. SADAT, SAFIEH AMINI, G. BAHRAMALI, F. ROUHVAND, A. BUDKOWSKA, A.R. KHABIRI, and S.R. NADAF, “EVALUATION OF A NATIVE PREPARATION OF HCV CORE PROTEIN (2-122) FOR POTENTIAL APPLICATIONS IN IMMUNIZATION, DIAGNOSIS AND MAB PRODUCTION,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 35, no. 1, pp. 1–10, 2006, [Online]. Available: https://sid.ir/paper/272227/en

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