MOLECULAR CHARACTERIZATION OF SUBUNIT G OF THE VACUOLAR ATPASE IN PATHOGEN DERMATOPHYTE TRICHOPHYTON RUBRUM

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REZAEI SASAN; KHODADADI H.; NOURBAKHSH F.; SAFARI M.R.

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Journal Paper

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Journal: Iranian Journal of Public Health Year:2006 | Volume:35 | Issue:2 Page(s): 33-37

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Title

MOLECULAR CHARACTERIZATION OF SUBUNIT G OF THE VACUOLAR ATPASE IN PATHOGEN DERMATOPHYTE TRICHOPHYTON RUBRUM

Author(s)

REZAEI SASAN | KHODADADI H. | NOURBAKHSH F. | SAFARI M.R. | Issue Writer Certificate

Keywords

DERMATOPHYTE

TRICHOPHYTON RUBRUM

FUNGAL RNA

FUNGAL DNA

IRAN

Abstract

Trichophyton rubrum is an anthropophilic fungus causing up to 90% of chronic cases of dermatophytosis. Several properties of this fungus have been investigated so far. However, a few studies were carried out in the field of molecular biology of this fungus. In the present study, we tried to identify the subunit G of its vacuolar ATPase (V-ATPase). Pairs of 21 nt primers were designed from highly conserved regions of the V-ATPase subunit G genes in other fungi. Mentioned primers were utilized in PCR using isolated genomic DNA template as well as cytoplasmic RNA of T.rubrum and the PCR and RT-PCR fragments were then sequenced. About 469 nucleotides were sequenced which encoded a polypeptide with 119 amino acids. Nucleotide sequence comparison in gene data banks (NCBI, NIH) for both the DNA and its deduced amino acid sequence revealed significant homology with V-ATPase subunit G genes and proteins of other eukaryotic cells. The amino acid sequence of the encoded protein was about 84% identical to the sequence of V-ATPase subunit G from other fungi. In summary, we have cloned the first V-ATPase subunit G of DERMATOPHYTEs and characterized it as a member of this gene family in other eukaryotic cells.

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APA: Copy

REZAEI, SASAN, KHODADADI, H., NOURBAKHSH, F., & SAFARI, M.R.. (2006). MOLECULAR CHARACTERIZATION OF SUBUNIT G OF THE VACUOLAR ATPASE IN PATHOGEN DERMATOPHYTE TRICHOPHYTON RUBRUM. IRANIAN JOURNAL OF PUBLIC HEALTH, 35(2), 33-37. SID. https://sid.ir/paper/272242/en

Vancouver: Copy

REZAEI SASAN, KHODADADI H., NOURBAKHSH F., SAFARI M.R.. MOLECULAR CHARACTERIZATION OF SUBUNIT G OF THE VACUOLAR ATPASE IN PATHOGEN DERMATOPHYTE TRICHOPHYTON RUBRUM. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2006;35(2):33-37. Available from: https://sid.ir/paper/272242/en

IEEE: Copy

SASAN REZAEI, H. KHODADADI, F. NOURBAKHSH, and M.R. SAFARI, “MOLECULAR CHARACTERIZATION OF SUBUNIT G OF THE VACUOLAR ATPASE IN PATHOGEN DERMATOPHYTE TRICHOPHYTON RUBRUM,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 35, no. 2, pp. 33–37, 2006, [Online]. Available: https://sid.ir/paper/272242/en

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