DIAGNOSIS AND CHARACTERIZATION OF LEISHMANIA SPECIES IN GIEMSA-STAINED SLIDES BY PCR-RFLP

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KAZEMIRAD E.; MOHEBALI M.; HAJARAN H.; REZAEI SASAN; MAMISHI S.

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Journal Paper

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Journal: Iranian Journal of Public Health Year:2008 | Volume:37 | Issue:1 Page(s): 54-60

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Title

DIAGNOSIS AND CHARACTERIZATION OF LEISHMANIA SPECIES IN GIEMSA-STAINED SLIDES BY PCR-RFLP

Author(s)

Keywords

LEISHMANIA SPECIES

GIEMSA-STAINED SMEAR

PCR-RFLP

Abstract

Background: Direct identification of LEISHMANIA SPECIES in Giemsa-stained slides without parasite culturing in the areas where multiple species exist, is very helpful. This study was designed to isolate Leishmani spp. from GIEMSA-STAINED SMEARs and to characterize them by PCR technique.Methods: A total of 48 Giemsa-Stained slides from confirmed cases of leishmaniasis were examined under a light microscope at×1000 and classified based on grading of Leishmania parasites. DNA from each slide was extracted separately and subjected to PCR. The ribosomal internal transcribed spacer 1 (ITS1) was amplified with specific primers and the PCR products were digested with a restriction enzyme (HaeIII).Results: Of the 48 microscopy-positive slides, 43(89.6%) were positive by PCR-RFLP and LEISHMANIA SPECIES were identified. A statistically significant difference was observed between the both methods (P< 0.05) and also a concordance was found between microscopy and PCR-RFLP (k= 0.55).Conclusion: PCR-RFLP seems to be an effective method to identify LEISHMANIA SPECIES from GIEMSA-STAINED SMEARs which have been collected from both infected humans and animal reservoir hosts in Iran.

Cites

DIAGNOSIS AND CHARACTERIZATION OF LEISHMANIA SPECIES IN PATIENTS AND RODENTS GIEMSA-STAINED SLIDES BY PCR-RFLP IN DAMGHAN DISTRICT, IRAN

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APA: Copy

KAZEMIRAD, E., MOHEBALI, M., HAJARAN, H., REZAEI, SASAN, & MAMISHI, S.. (2008). DIAGNOSIS AND CHARACTERIZATION OF LEISHMANIA SPECIES IN GIEMSA-STAINED SLIDES BY PCR-RFLP. IRANIAN JOURNAL OF PUBLIC HEALTH, 37(1), 54-60. SID. https://sid.ir/paper/272471/en

Vancouver: Copy

KAZEMIRAD E., MOHEBALI M., HAJARAN H., REZAEI SASAN, MAMISHI S.. DIAGNOSIS AND CHARACTERIZATION OF LEISHMANIA SPECIES IN GIEMSA-STAINED SLIDES BY PCR-RFLP. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2008;37(1):54-60. Available from: https://sid.ir/paper/272471/en

IEEE: Copy

E. KAZEMIRAD, M. MOHEBALI, H. HAJARAN, SASAN REZAEI, and S. MAMISHI, “DIAGNOSIS AND CHARACTERIZATION OF LEISHMANIA SPECIES IN GIEMSA-STAINED SLIDES BY PCR-RFLP,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 37, no. 1, pp. 54–60, 2008, [Online]. Available: https://sid.ir/paper/272471/en

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