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Title

INVESTIGATION OF VARIOUS TISSUE CULTURE MONOLAYERS SENSITIVITY IN DETECTION OF CLOSTRIDIUM DIFFICILE TOXIN

Pages

  99-102

Abstract

 Background: CLOSTRIDIUM DIFFICILE is the most common cause of nosocomial DIARRHEA. It is usually a consequence of antibiotic treatment, but sporadic cases can occur. The purpose of this study was to investigate five tissue culture monolayers sensitivity in detection of C. difficile-toxin.Methods: A total of 402 stool samples from PATIENTS with nosocomial DIARRHEA hospitalized in three hospitals of Tehran University of Medical Sciences (TUMS) were collected. The samples were cultured on a selective cycloserine cefoxitin fructose agar (CCFA) and incubated in anaerobic conditions, at 37°C for 4 days. Isolates were characterized to species level by conventional biochemical tests. Bacterial cytotoxicity was assayed on five tissue culture monolayers.Results: Our findings show that of the total PATIENTS, 24 toxigenic C. difficile (6%) were isolated. All 24 C. difficile TOXINs showed cytotoxic effect at ≥ 1:10 dilution on Hela, Hep2, Vero, McCoy and Mdck cells after 16, 20, 24, 24 and 30 hours, respectively. C. difficile TOXIN showed cytotoxic effect at ≥ 1:100 dilutions only on Hela cell monolayer after 48 hours. Conclusion: Hela cell monolayer may be a satisfactory substitute for the detection of C. difficile TOXIN in clinical specimens.

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    APA: Copy

    SALARI, M.H., BADAMI, N., SADEGHIFARD, NOUR KHODA, & AMIN HARATI, F.. (2008). INVESTIGATION OF VARIOUS TISSUE CULTURE MONOLAYERS SENSITIVITY IN DETECTION OF CLOSTRIDIUM DIFFICILE TOXIN. IRANIAN JOURNAL OF PUBLIC HEALTH, 37(1), 99-102. SID. https://sid.ir/paper/272475/en

    Vancouver: Copy

    SALARI M.H., BADAMI N., SADEGHIFARD NOUR KHODA, AMIN HARATI F.. INVESTIGATION OF VARIOUS TISSUE CULTURE MONOLAYERS SENSITIVITY IN DETECTION OF CLOSTRIDIUM DIFFICILE TOXIN. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2008;37(1):99-102. Available from: https://sid.ir/paper/272475/en

    IEEE: Copy

    M.H. SALARI, N. BADAMI, NOUR KHODA SADEGHIFARD, and F. AMIN HARATI, “INVESTIGATION OF VARIOUS TISSUE CULTURE MONOLAYERS SENSITIVITY IN DETECTION OF CLOSTRIDIUM DIFFICILE TOXIN,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 37, no. 1, pp. 99–102, 2008, [Online]. Available: https://sid.ir/paper/272475/en

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