مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

DETERMINATION OF HBV GENOTYPES AMONG HBS AG POSITIVE BLOOD DONORS IN TEHRAN, IRAN USING PCR-RFLP

Pages

  41-47

Abstract

 Background: Hepatitis B virus (HBV) is one of the major causative agents of acute and chronic liver disease worldwide and is believed to be responsible for a million deaths annually. On the basis of a comparison of complete genomic sequences, HBV has been classified into eight genotypes A-H which show a geographical distribution. Some genotypes are associated with different clinical outcomes. Identification of HBV genotypes is important to begin and follow up the treatment. Methods: In this cross-sectional study, the serum samples of BLOOD DONORS were collected from Tehran Blood Transfusion Centers in period during "2005-2006". Sera of 55 BLOOD DONORS who were positive for hepatitis B surface antigen were selected. DNA was extracted using commercial kit and the S gene sequence was amplified by nested-PCR. PCR products were then analyzed for restriction enzymes that would be genotype specific.Results: Genotype D was found the only type in all HBV DNA positive serum samples, in Tehran.Conclusion: Genotype D is dominant among Tehran’s BLOOD DONORS, which is consistent with IRAN and the Middle East dominant genotype.

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    • Cite

    APA: Copy

    MILANI, S., SHARIFI, Z., HOSSEINI, M., & MAHMOUDIAN SHOUSHTARI, M.. (2009). DETERMINATION OF HBV GENOTYPES AMONG HBS AG POSITIVE BLOOD DONORS IN TEHRAN, IRAN USING PCR-RFLP. IRANIAN JOURNAL OF PUBLIC HEALTH, 38(1), 41-47. SID. https://sid.ir/paper/272561/en

    Vancouver: Copy

    MILANI S., SHARIFI Z., HOSSEINI M., MAHMOUDIAN SHOUSHTARI M.. DETERMINATION OF HBV GENOTYPES AMONG HBS AG POSITIVE BLOOD DONORS IN TEHRAN, IRAN USING PCR-RFLP. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2009;38(1):41-47. Available from: https://sid.ir/paper/272561/en

    IEEE: Copy

    S. MILANI, Z. SHARIFI, M. HOSSEINI, and M. MAHMOUDIAN SHOUSHTARI, “DETERMINATION OF HBV GENOTYPES AMONG HBS AG POSITIVE BLOOD DONORS IN TEHRAN, IRAN USING PCR-RFLP,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 38, no. 1, pp. 41–47, 2009, [Online]. Available: https://sid.ir/paper/272561/en

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