PLASMODIUM VIVAX MSP-3β GENE AS A GENETIC MARKER FOR THE PARASITE DETECTION IN COMPARISON WITH SSRRNA GENE

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SHAHBAZI A.; MIRHENDI H.; RAEISI A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

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Journal: Iranian Journal of Public Health Year:2010 | Volume:39 | Issue:2 Page(s): 105-109

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Title

PLASMODIUM VIVAX MSP-3β GENE AS A GENETIC MARKER FOR THE PARASITE DETECTION IN COMPARISON WITH SSRRNA GENE

Author(s)

SHAHBAZI A. | MIRHENDI H. | RAEISI A. | Issue Writer Certificate

Keywords

PVMSP-3β

PLASMODIUM VIVAX

SSRRNA

IRAN

Abstract

Background: The importance of accurate diagnosis of all of major diseases cannot be underestimated and efficient laboratory testing is vital to identifying and treating life-threatening illnesses including malaria. In this study, we compared the potential of one of merozoite surface protein genes, PvMSP-3b, for detection of PLASMODIUM VIVAX in blood samples by PCR with routinely used marker, SSRRNA gene.Methods: One hundred P. vivax microscopy-positive blood samples were simultaneously tested with two genetic markers, including PvMSP-3b gene and SSRRNA gene by PCR and nestedPCR method, respectively, and their sensitivity and specificity in detection of P. vivax was compared.Results: An important difference was seen in sensitivity between the 2 genetic markers, 100% in case of SSRRNA gene vs. 95% of PvMSP-3b gene. The specificity of the two markers was same (100%). Microscopic diagnoses of thick and thin blood smears was used as "golden standard" method.Conclusion: Due to critical importance of accurate detection of the parasite in malarious area, the PvMSP-3b gene cannot be a suitable marker for detection of P. vivax in blood sample by PCR. More investigations are needed to find other valid markers.

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APA: Copy

SHAHBAZI, A., MIRHENDI, H., & RAEISI, A.. (2010). PLASMODIUM VIVAX MSP-3β GENE AS A GENETIC MARKER FOR THE PARASITE DETECTION IN COMPARISON WITH SSRRNA GENE. IRANIAN JOURNAL OF PUBLIC HEALTH, 39(2), 105-109. SID. https://sid.ir/paper/272722/en

Vancouver: Copy

SHAHBAZI A., MIRHENDI H., RAEISI A.. PLASMODIUM VIVAX MSP-3β GENE AS A GENETIC MARKER FOR THE PARASITE DETECTION IN COMPARISON WITH SSRRNA GENE. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2010;39(2):105-109. Available from: https://sid.ir/paper/272722/en

IEEE: Copy

A. SHAHBAZI, H. MIRHENDI, and A. RAEISI, “PLASMODIUM VIVAX MSP-3β GENE AS A GENETIC MARKER FOR THE PARASITE DETECTION IN COMPARISON WITH SSRRNA GENE,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 39, no. 2, pp. 105–109, 2010, [Online]. Available: https://sid.ir/paper/272722/en

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