ISOLATION AND IDENTIFICATION OF RAINMAKING BACTERIA

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AMIRZADEH SHAMS SHOKOOFEH; KHANAFARI ANITA

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Journal: Iranian Journal of Public Health Year:2014 | Volume:43 | Issue:SUPPLEMENT 2 Page(s): 133-133

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Title

ISOLATION AND IDENTIFICATION OF RAINMAKING BACTERIA

Author(s)

AMIRZADEH SHAMS SHOKOOFEH | KHANAFARI ANITA

Keywords

BIOPRECIPITATION

PSEUDOMONAS SYRINGE

DROPLET FREEZING METHOD

INA BACTERIA

Abstract

Background: Ice nucleation active (INA) bacteria have play a role in atmospheric processes and participate in assort of biological precipitation cycle. The most surveys on these bacteria were from subtropical area, so, studies of INA BACTERIA from other area are needed. The aim of current research was isolation and identification of INA BACTERIA from north of Tehran, Iran to investigate their ice nucleation property.Methods: Bacterial genus were isolated from plant surface (Japan allspice) and winter precipitation (snow and rain) after culturing on BHI medium and incubated in a various temperatures (4, 10, 15, 20 and 25oC) for 24-96 hours. DROPLET FREEZING METHOD was used to screen potential of ice nucleation producing bacteria. 16S rRNA analysis was applied to confirm genus and species of the isolates. Outer membrane protein of ice nucleation positive strain was purified and identified by SDS-PAGE method.Results: The results showed that among 50 isolates, 40 strains had the ability to produce ice nucleation and 10 strains showed extremely high ice nucleation activity. Gram positive cocobacilli (5%), gram negative bacilli and gram positive cocci (25%) were determined as the least and the most microbial populations, respectively.Conclusion: The best strain was a gram-negative rod shape with the ability of complete freezing of water in less than 5 minutes. It was identified as PSEUDOMONAS SYRINGEa based on 16S rRNA gene sequencing. Selected isolate in this research could be a promising tool for biopercipitation.

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APA: Copy

AMIRZADEH SHAMS, SHOKOOFEH, & KHANAFARI, ANITA. (2014). ISOLATION AND IDENTIFICATION OF RAINMAKING BACTERIA. IRANIAN JOURNAL OF PUBLIC HEALTH, 43(SUPPLEMENT 2), 133-133. SID. https://sid.ir/paper/273498/en

Vancouver: Copy

AMIRZADEH SHAMS SHOKOOFEH, KHANAFARI ANITA. ISOLATION AND IDENTIFICATION OF RAINMAKING BACTERIA. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2014;43(SUPPLEMENT 2):133-133. Available from: https://sid.ir/paper/273498/en

IEEE: Copy

SHOKOOFEH AMIRZADEH SHAMS, and ANITA KHANAFARI, “ISOLATION AND IDENTIFICATION OF RAINMAKING BACTERIA,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 43, no. SUPPLEMENT 2, pp. 133–133, 2014, [Online]. Available: https://sid.ir/paper/273498/en

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